402 BACTERIOLOGY. 



needle, and after seventy-two and ninety-six hours the 

 appearances shown in Fig. 80, c and d, will be produced. 



There is nothing particularly characteristic about its 

 growth upon agar-agar. 



On potato there appears a moist, glazed, yellowish, 

 and, at points, brownish-yellow growth that is sur- 

 rounded by a drier, colorless zone. It is not lobu- 

 lated. 



In milk containing neutral litmus tincture a pink color 

 appears after two to three days at 37 C. ; after four 

 days the milk is almost decolorized and there is begin- 

 ning to appear coagulation of the casein with a layer of 

 clear whey above it. During the subsequent twenty- 

 four hours there is complete separation of the contents 

 of the tube into clot and whey. 



In Dunham's peptone solution it does not form indol, 

 and the reaction for this body does not appear with 

 either sulphuric acid alone or plus sodium nitrite. 



It causes liquefaction of both coagulated blood-serum 

 and egg-albumin. 



There is no pellicle formed as a result of its growth 

 in bouillon. 



It does not produce fermentation of glucose. 



In rosolic-acid-peptone solution its growth causes the 

 red color to become deepened after four or five days at 

 37 C. 



By Koch's method of introducing cultures into the 

 stomachs of guinea-pigs this organism produced the 

 death of three out of fifteen animals experimented 

 upon the deaths resulting, most probably, more from 

 the toxic action of the products of growth that were 

 introduced with the organisms than to any pathogenic 

 powers possessed by the organism itself. 



