516 BACTERIOLOGY. 



which had been immersed in solutions of sublimate 

 gave the characteristic reactions of the salt after having 

 been immersed in distilled water, which had been re- 

 peatedly renewed, for five weeks. 



He remarks that a similar firm combination between 

 sublimate and cotton will take place after a longer time, 

 but it occurs so slowly that it cannot interfere with dis- 

 infection experiments in the same way as he believes the 

 employment of silk to act. 



The most successful attempt at removing all traces of 

 sublimate from the threads or from the proteid sub- 

 stances in which are located the bacteria whose vitality 

 is to be tested is that made by Geppert, who subjected 

 them to the action of ammonium sulphide in solution. 

 By this procedure the mercury is converted into insolu- 

 ble sulphide, and does not now have an inhibiting effect 

 upon the growth of those bacteria that may not have suc- 

 cumbed to its action when in the form of the bichloride. 



In the second method of testing disinfectants, men- 

 tioned above that is, when cultures of bacteria and 

 solutions of the disinfectant are mixed, and after a time 

 a drop of the mixture is removed and added to sterile 

 nutrient media, the inhibiting amount of disinfectant 

 can readily be gotten rid of by dilution that is to say, 

 instead of transporting the drop directly to the fresh 

 medium, add it to 10 or 12 c.c. of sterilized salt-solu- 

 tion (0.6-0.7 per cent, of NaCl in distilled water), or 

 distilled water, and after thoroughly shaking add a drop 

 of this to the medium in which the power of develop- 

 ment of the bacteria is to be determined. 



Another important point to be borne in mind in test- 

 ing disinfectants is the necessity of so arranging the 

 conditions that each individual organisms will be ex- 



