32 STUDENTS HISTOLOGY 



cially recommended as a nuclear staiii. The sections are treated 

 according to the programme just given, except that they should 

 remain in the safranin solution (one per cent.) some hours. 

 Safranin has been employed largely in staining karyokinetic 

 figures, after hardening with Flemming's solution (page 23). 



NIGROSIN is an aniline color used mostly for nervous tissues. 

 It is valuable when results are desired at once, without waiting 

 for the tedious hardening process required for the Weigert-Pal 

 method. 



a. Stain sections in strong watery solution of nigrosin five to 

 ten minutes. 



&. Wash and mount in the usual way in balsam, clearing in 

 xylol. 



VAN GIBSON'S STAIN 



Acid fuchsin (one per cent, watery solution) . . . 15 c.c. 



Picric acid (saturated watery solution) 50 c.c. 



Water 50 c.c. 



a. Stain sections in haematoxylin. 

 &. Wash in water. 



c. Stain in the picric acid and acid fuchsin from three to five 

 minutes. 



d. Wash in water and mount in the usual way in balsam. 



The Van Gieson stain is used chiefly for connective and nervous 

 tissues. 



EHRLICH TRICOLOR STAIN 



This formula is only one of a number with which Ehrlich's 

 name is associated, as well as those of Biondi and Heidenhain. A 

 powder containing the dyes already mixed is sold by dealers, and 

 usually works very well. It may be used to stain sections of 

 tissues, but is employed mostly with preparations of blood, dried 

 on cover -glasses and fixed lay heating. Stain five minutes or less. 

 It is designed to stain the neutrophile granules of certain leucocytes, 



