BORAX- CARMINE STAINING PROCESS 



39 



pink, and it affords with the ha3matoxylin a good contrasting color. 

 The tissue is to be stained in haBmatoxylin and washed in water as 

 usual ; then it is placed in the eosin solution, and afterward 

 washed again. The subsequent treatment is as with the plain 

 hromatoxyliii process; viz., dehydration with alcohol, after whicji 

 the oil of cloves. 



The diagram, Fig. 18, shows the process complete: 



1. Watch-glass with ha3matoxylin. 



2. Saucer with water. 



3. Watch-glass two -thirds filled with water, with five drops of 

 eosin solution added. 



4. Saucer containing water. 



5. Saltcellar filled with alcohol. 



6. Covered oil -dish. 



The eosin stains very quickly, generally in about a minute. 

 Care should be taken not to overstain with it, as it cannot be 



FIG. 18. 



DIAGRAM INDICATING THE SUCCESSIVE STEPS IN DOUBLE STAINING WITH 

 H^EMATOXYI.IN AND EOSIN. 



washed out. If the sections are found at any time to be over- 

 stained with haematoxylin the color may be removed to any desired 

 extent by floating them in a weak solution of acetic acid. They 

 must afterward be washed very thoroughly in clean water. 



BORAX -CARMINE STAINING PROCESS 



Arrange your materials as in the diagram, Fig. 19. 



1. Watch-glass two -thirds filled with the carmine fluid. 



2. Saucer containing about an ounce of alcohol. 



3. Saltcellar filled with alcohol containing one per cent, of 

 hydrochloric acid. 



4. Saltcellar with alcohol. 



5. Porcelain dish containing oil of cloves. 



The carmine solution will stain ordinarily in fifteen or twenty 

 minutes. After the section has been in the dye for a few minutes, 



