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STUDENTS HISTOLOGY 



PAROTID AND SUBMAXILLARY GLANDS, AND THE PANCREAS 

 Practical Demonstration 



The tissue must be fresh, divided in small pieces not larger than a centi- 

 meter cube and hardened by placing in ninety-five per cent, alcohol for twelve 

 hours, after which fresh spirit should be substituted. If, after the lapse of 

 another twelve hours, the tissue should not be sufficiently firm, it should be 

 placed in a small quantity of absolute alcohol for three hours. Sections should 



Fia. 99. SECTION FROM THE PANCREAS. 



A. Wall of a large duct. 



B. The somewhat cubical lining cells. 



C. Arteries. 



D. Lumen of the acini, T. S. 



E. Terminal duct leaving a lobule. 



F. Acini in L. S. 



be made immediately after hardening as more prolonged action of the strong 

 spirit will cause the tissue to contract. 



Sections may be cut with or without a simple microtome the desideratum 

 being thin rather than large cuts. 



Stain lightly with haematoxylin and deeply with eosin. 



After sections of hardened tissue have been examined, the glandular 



