170 STUDENTS HISTOLOGY 



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these pocket -like glands does not pass directly into the lumen of 

 the duct, but runs along obliquely, much like glands in the 

 bronchi. Not infrequently the glands possess no proper efferent 

 tube, but are mere depressions or diverticula in the thick wall of 

 the bile-duct.) 



13. The lobular parenchyma. (Single cells, partly detached, 

 may be found about the edges of the section.) Note: (a) The 

 somewhat polygonal figure; (&) the nucleus; (c) nucleoli ; 

 (d) fibrillated, mesh-like cell-body; and (e) an apparent cell-wall. 

 (The arrangement of the lobular parenchyma will be noted in con- 

 nection with the human liver.) 



HUMAN LIVER 

 PRACTICAL DEMONSTRATION 



The sections from which the illustrations have been drawn were made from 

 material hardened in Miiller's fluid. The tissue was then cut, the sections 

 washed by six hours' maceration in water, after which they were treated suc- 

 cessively with weak and stronger alcohol, stained with hsematoxylin and eosin, 

 and mounted in balsam. This treatment aids greatly in the demonstration of 

 the blood-capillaries, as the contained blood-corpuscles, in consequence of 

 some change affected by the chromium salt, take the eosin deeply. The 

 nuclei of cells are also rendered markedly prominent. 



Pieces of tissue, one centimeter square by half a centimeter thick, may be 

 hardened in alcohol. This method will give very excellent results, providing 

 the sections be cut as soon as the hardening process has become complete. 

 Stain as above. 



For the demonstration of the isolated hepatic cells, scrape the cut surface 

 of a piece of hardened liver with a scalpel, and throw the scrapings into a 

 watch-glass of hasmatoxylin. After a few moments drain off the stain, and 

 brush the stained tissue elements into a test-tube nearly filled with water. 

 Change the water two or three times ; and when clear, add a few drops of eosin 

 solution. Allow the eosin to stain for a moment only; decant, drain, and fill 

 the tube with alcohol. After ten minutes the spirit may be drained off and 

 the tube partly filled with oil of cloves. A drop of the sediment may then 

 be placed upon the slide, the bulk of the oil removed with paper, and the 

 mounting completed by adding a drop of balsam and the cover-glass. This 

 tissue may be kept in the oil from year to year for class-room purposes. If 

 the oil be pure and the washing thorough, the staining will remain unaffected 

 for two or three years. 



