240 STUDENTS HISTOLOGY 



VERTICAL SECTION OF HUMAN CEREBELLUM 

 Practical Demonstration 



Cut large sections of cerebellum, hardened with Miiller's fluid and im- 

 bedded as usual ; cut so as to show the ramifications of the arbor vita? ; stain 

 with haematoxylin and eosin. Also stain the cerebellum of a young dog or 

 kitten, prepared according to Golgi's method (page 33), being careful to cut 

 the sections at a right angle to the long axis of the convolutions. 



OBSERVE : ( Fi s - 156 and 157 > 



(L.) 



1. The arrangement in the form of leaflets. 



2. The extension of the gray laminae within even the mi- 

 nutest folds of the leaves, so as to completely envelop the central 

 white nerve -substance. (The staining has been so selected by the 



FIG. 156. LONGITUDINAL SECTION OF ONE OF THE FOLIA OF THE CEREBELLUM (X60). 



A, A. Line of pia mater. 



B, B. Sulci. 



C, C. Outer layer of gray matter. 



D, D. Inner layer of gray matter, including Purkinje's cells. 



E, E. White nerve-substance. 



