(Triacid Stain.) 



i, 2, 3, 4. Small Lymphocytes. 



Contrast the faintly colored protoplasm of these cells in the triple stained specimen, 

 with their intensely basic protoplasm in the film stained with eosin and methylene- 

 blue, 17 and 18. 'The cell body of i is invisible. Note the kidney-shaped nucleus 

 in 4. 



5, 6. Large Lymphocytes. 



With this stain the nucleus reacts more strongly than the protoplasm; with eosin 

 and methylene-blue (19, 20), on the contrary, the protoplasm is so deeply stained 

 that the nucleus appears pale by contrast. This peculiarity is also observed in 

 the smaller forms of lymphocytes. 



7, 8. Transitional Forms. 



Note the moderately basic and indented nucleus, and the almost hyaline non-granular 

 protoplasm. Compare 8 with the myelocyte, 7, Plate I, these cells differing chiefly 

 in that the myelocyte contains neutrophile granules. 



9, 10, ii. Polynuclear Neutrophiles. 



These cells are characterized by a polymorphous or polynuclear nucleus, surrounded 

 by a cell-body filled with fine neutrophile granules. In n the nuclear structure is 

 obviously separated into four parts; in 9 it is moderately, and in 10 markedly, poly- 

 morphous. 



12, 13. Eosinophiles. 



The nuclei are not unlike those of the polynuclear neutrophile, except that they are 

 somewhat less convoluted, and poorer in chromatin, staining less intensely. The 

 protoplasm is filled with coarse eosinophile granules, the characteristics of which 

 are clearly illustrated by 13, a "fractured" eosinophile. 



14. Eosinophilic Myelocyte. 

 Compare with 15. 



15, 1 6. Myelocytes. (Neutrophili-c.) 



These cells are morphologically similar to 14, except that they contain neutrophile 

 instead of eosinophile granules. Note that the granules of the myelocyte are identical 

 with those of the polynuclear neutrophile. A dwarf form of myelocyte is repre- 

 sented by 1 6. 



{Eosin and Methylene-bhie.) 



17, 1 8. Small Lymphocytes. 



Note the narrow rim of pseudo-granular basic protoplasm surrounding the nucleus, 

 and the pale appearance of the latter. 



19, 20. Large Lymphocytes. 



Budding of the basic zone of protoplasm is represented by 20. Both of these cells 

 belong to the same type as 5 and 6. 



21, 22. Large Mononuclear Leukocytes. 



Compared with 19 and 20, these cells have a decidedly less basic protoplasm, but 

 a somewhat more basic nucleus. In the triple stained film these differences can- 

 not be detected, so that they must be classed as large lymphocytes. 



23. Transitional Form. 



The distinction between this cell and 24 is not marked; the nucleus of the latter 

 simply being somewhat more basic and convoluted. 



24, 25, 26, 27. Polynuclear Neutrophiles. 



With this stain these cells show a feebly acid protoplasm, and lack granules. Note 

 that the more twisted the nucleus the deeper it is stained. Compare with 9, 10 

 and ii. 

 28, 29. Eosinophiles. 



Compare with 12 and 13. 



30. Eosinophilic Myelocyte. 

 Compare with 14 



31. Basophile. (Finely granular.') 



This cell is characterized by the presence of exceedingly fine 5-granules, staining 

 the pure color of the basic dye. The nucleus is markedly convoluted and deficient 

 in chromatin. The cell here shown was found in normal blood. 



3 2 . 33. 34, 35, 3 6 - Mast Cells. 



The granules take a modified basic color, as shown by their royal-purple tint in this 

 illustration. Note their unusually large size and ovoid shape in 35, their peculiar 

 distribution in 35 and 36, and their irregularity in size in 32 and 36. With the triacid 

 mixture these granules, as well as those of the finely granular basophile, 31, remain 

 unstained, showing as dull-white stippled areas in the cell-body. The nuclear chro- 

 matin of the mast cell is so delicate and so feebly stained that it is barely visible. 

 These cells were found in the blood of a case of splenomedullary leukemia. 



