CHAP. III.] LYSATININE OR LYSATINE ? 257 



this mixture five or six volumes of alcohol and some ether it becomes very 

 turbid, and subsequently, as the turbidity disappears, a thick oil falls to the 

 bottom of the vessel in which the precipitation is effected. The clear 

 mother liquor is now poured off and gradually treated with ether in small 

 quantities, until a separation of crystals, which adhere to the walls of the 

 vessel, commences : at this stage a large excess of ether is added and the 

 mixture set aside for the night in a cool place. The following day snow- 

 white needles and flakes are found to have separated, which are recrys- 

 tallised from a small quantity of water, to which alcohol and ether are 

 added, and are thus obtained perfectly pure 1 . 



Siegfried's From the precipitate which phosphotungstic acid pro- 



method of se- duces when added to the products of decomposition of the 

 parating lysine proteids, lysine and lysatinine may be readily separated, 

 and lysatinine The precipitate having been freed from chlorine, it is dis- 

 directly from solved (almost completely) in boiling water and decomposed 

 the phospho- by means of the smallest possible excess of Ba(OH) 2 . The 

 ungstic^ pre- fii tra t e from the barium precipitate is saturated with CO 2 , 

 boiled for half-an-hour, filtered and, when cold, treated with 

 solution of silver nitrate, so long as a precipitate falls. The voluminous 

 precipitate is separated and thoroughly washed with water. 



The filtrate from the silver precipitate is concentrated to the consistence 

 of a thin syrup and then, little by little, treated with small quantities of 

 absolute alcohol, which causes a somewhat dense precipitate, which assumes 

 an obscurely crystalline form and which contains lysine. (From this pre- 

 cipitate the platinochloride of lysine may be obtained, in a pure condition, 

 by decomposing by means of H 2 S, concentrating the filtrate, treating with 

 PtCl 4 , then with alcohol and ether in the manner described at p. 255.) 



The filtrate from which the smeary compound of lysine and silver has 

 separated through the gradual addition of alcohol, is still further treated 

 with alcohol, when the tine crystalline compound of lysatinine and silver 

 nitrate is obtained, exactly as described previously. 



Composition The analysis of the silver compound leads to the 



of the silver formula C 6 H 1S N 8 O* . HON0 2 + AgO . N0 2 , from which 

 compound of however probably is to be subtracted a molecule of 

 water of crystallisation. 



From the empirical formula derived from the analysis 

 ^ tne previously described compound, lysatinine is seen 

 to have the composition of a creatine or a creatinine, 

 according as we assume that one molecule of water is or is not water of 

 crystallisation. Its composition in the latter case would be expressed 

 by the formula C n H 2n+1 N 3 O 2 , which is also the empirical formula of 

 creatine, C H H 8B+1 N 8 O a ; in that case we should term it lysatine. 

 Creatine ... C 4 H 9 N 3 2 . 

 Lysatine ... C 6 H 13 IS 3 O 2 . 



1 The details of the process are taken from Ernst Fischer's paper, ' Ueber neue 

 Spaltungsproducte des Leimes.' Extract from 'Inaugural Dissert., ' Leipzig, 1890, in 

 Drechsel's memoir, 'Der Abbau der Eiweissstoffe." 



2 Siegfried, ' Zur Kenntniss der Spaltungsproducte der Eiweisskorper,' see note 2, 

 p. 254. 



G. 17 



