332 



CHOLETELIN. CHOLOH^EMATIN. 



[BOOK IL 



Hydrobilirubin. 



Fluorescent properties Exhibits a green fluor- 

 of an ammoniacal escence. 

 solution containing 



zinc. 



Choletelin. 



Colour of alkaline solu- Brownish-yellow to yel- Yellow, 

 tion. low, according to con- 



centration. 



No fluorescence. 



Reaction when treated 

 with one drop of 

 sulphuric acid and 

 a tiny grain of salt- 

 petre (Liebermann). 



Exhibits the variegated None, 

 colours characteristic 

 of Gmelin's reaction. 



SECT. 10. IMPERFECTLY INVESTIGATED COLOURING MATTERS NOT 

 PREEXISTENT IN BlLE, BUT DERIVED FROM CflROMOGENS EXIST- 

 ING IN IT. 



1. Cholohwmatin. 



When the bile of the sheep or of the ox is examined some time 

 after death, especially after it has been well shaken with air, it 

 usually presents an absorption spectrum which is characterised by 

 the presence of four absorption bands (see Plate II. fig. 1) the 

 position of which will, in the sequel, be indicated. 

 Hoppe- I n the third edition of his practical handbook, 



published in 1870, Hoppe-Seyler 1 speaking of the bile 

 observations. r f ,, -11 , i i /> i c 



of the ox described it as possessed, when fresh, of a 



green colour and exhibiting, when tolerably thick layers are examined 

 with the spectroscope, an absorption band between D and E, but 

 nearer D. He, however, asserted that when such bile is kept it 

 exhibits a spectrum, also observed in the case of the alcoholic 

 solution of evaporated ox-bile, marked by 4 absorption bands, of 

 which one is close to C, a second between G and D but closer to D, 

 a third between D and E but closer to D, and a "fourth in the 

 proximity of E. This author remarked that the colouring matter 

 which occasions this spectrum is also found in sheep's bile, but that 

 nothing was known in regard to it. 



The obser- In 1871, in their already quoted paper, Heynsius 



vations of and Campbell gave an admirable and in all respects 



Heynsius and accura te plate 2 of the spectrum observed on examining 



an alcoholic solution of ox-bile. They made the most 



1 F. Hoppe-Seyler, Handbuch der phys. u. path, chemisch. Analyse, Dritte Aufl. 

 Berlin, 1870, p. 182. 



2 A. Heynsius and J. F. F. Campbell, ' Die Oxydationsproducte der Gallenfarbstoffe 



