CHAP. V.] THE LYMPH AND CHYLE. TRANSLATIONS. 237 



ascertained by washing the coagulum from a known weight of the 

 transudation and proceeding as stated at page 180. 



The fibrinogen may then be determined by one of two methods : 

 firstly, by Frederique's method (see p. 188) ; secondly, by adding to a 

 weighed quantity of the liquid separated from any coagulum some 

 very active solution of fibrin-ferment (see p. 49), and then placing for 

 36 hours in an incubator heated to 40 C. ; then, collecting any 

 coagulum which has separated, washing, and proceeding as directed 

 in the case of blood-fibrin at p. 180. In giving the results of the 

 analyses the amount of fibrin, corresponding to fibrinogen, is then 

 stated. 



After separating fibrin and fibrinogen, the globulins remaining in 

 solution are estimated by Hammarsten's method (precipitation with 

 magnesium sulphate, see p. 188). 



In a fresh portion of the fluid the total proteids are estimated by 

 precipitation with alcohol (see p. 187). 



By then subtracting from the result thus obtained the weight of 

 fibrinogen and of globulins, the amount of serum-albumin is ascer- 

 tained. 



The extractive matters, salts and gases, are determined exactly 

 as in the case of blood or serum. 



