Reprinted from THE JOURNAL OF BIOLOGICAL CHEMISTRY 

 Vol. LIV, No. 1, September, 1922 



A STUDY OF THE DISTRIBUTION OF IODINE BETWEEN 

 CELLS AND COLLOID IN THE THYROID GLAND. 



IV. THE DISTRIBUTION OF IODINE IN THE HYPERPLASTIC THY- 

 ROID GLAND OF THE DOG AFTER THE INTRAVENOUS 

 INJECTION OF IODINE COMPOUNDS. 



BY HARRY BENJAMIN VAN DYKE. 



(From the Laboratories of Pharmacology, University of Chicago, Chicago.) 



(Received for publication, July 20, 1922.) 



Some years ago Marine and Feiss (1) and Marine and Hogoff 

 (2, 3) first performed experiments which leave little doubt as to the 

 ability of the dog's thyroid gland, especially when hyperplastic, 

 to bind iodine almost instantaneously. Marine and Feiss (1) 

 carefully perfused the surviving thyroid gland with fluid con- 

 taining iodine as KI. They found that after 1 hour a consider- 

 able amount of iodine was taken up only by a surviving gland. 

 Any evidence of death of the perfused organ was accompanied by 

 a loss of some of the gland's stored iodine rather than by an 

 absorption of iodine from the circulating medium. However, even 

 a surviving gland, rich in iodine, lost some iodine to a perfusing 

 fluid free of the element. In surviving glands they discovered 

 that the amounts of iodine absorbed were relatively independent 

 of the amounts of iodine in the perfusing fluids. They also pointed 

 out that a similarly rapid absorption of iodine by the intact gland 

 follows the intravenous administration of a solution of KI. From 

 the results of perfusions of spleen and kidney under similar condi- 

 tions they concluded that these organs were not capable of taking 

 up a significant amount of iodine. 



Marine and Rogoff (2) on the basis of experiments in which 

 they injected a solution of KI intravenously came to the con- 

 clusion that the absorption of iodine by the gland is almost as great 

 1 hour after the injection as it is 30 hours after the injection. 

 They again found that no significant amount of iodine was taken up 

 by the spleen and liver. The amount of iodine absorbed by the 



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