H. B. van Dyke 



327 



Finally autolysis does not seem to be much of a factor in the 

 loss if any occurs of iodine compounds from cells as they lie 

 in contact with Ringer's solution. Throughout the process of 

 cutting and separating the cells, the reaction of the suspending 

 medium should remain weakly alkaline a reaction which has 

 been shown to be unfavorable to autolysis. 4 Also in experiments 

 to be reported later in which the same technique was used under 

 slightly different conditions, every effort was made to eliminate 

 autolysis by cutting successively small portions of the gland and 

 floating the cells on Ringer's solution cooled by ice. The cells 

 from the small portions cut successively were at once centrifu- 

 galized and dried. No change could be noticed in the iodine 

 distribution. 



Results of Study of Dog Glands Taken at Random. 



TABLE II. 



Quantitative Determination of Iodine in Whole Gland and in Cells Free from 

 Colloid Material of Thyroid Glands of Normal Dogs. 



* No histological examination. 



4 Bradley, H. C., J. BioL Chem., 1915, xxii, 113. Bradley, H. C., and 

 Taylor, J., /. BioL Chem., 1916, xxv, 261. 



