86 CONTAGIOUS DISEASES OF DOMESTICATED ANIMALS. 



pig that had died from tlie result of inoculation with a very fatal virus 

 received fronriUinois. This cultivation liquid contained only micro- 

 cocci, the appearance of which are very well shown in Plate XI, Avhicli 

 was reproduced from a photograi)h. 



June 14, all had elevated temperatures varying from 104° to lOSf^ F., 

 increased thirst, tucked up abdomens, swelling at the points of inocula- 

 tion, rigors, and secluded themselves in their bedding. The appetite 

 was still fair. 



June 20, there was complete loss of fjppetite, emaciation, and profuse 

 diarrhea. 



June 2!), Xo. 27 died, and autopsy revealed congestion of intestines, 

 hei)atization of right lung, with abundant effusion in the pleural, peri- 

 cardial, and peritoneal cavities. Inoculations with this effusion caused 

 death of another pig July 8, after showing the w^ell-known symptoms of 

 swine plague. 



July 3, No. 28 was found in a dying condition and was destroyed, in 

 order to get fresh material for examination and for inoculation experi- 

 ments. 



July 6, No. 26 died in convulsions after having presented the charac- 

 teristic symptoms of the various stages of swiue plague. 



The notable point in this experiment is the virulence of the cultivated 

 virus. This virus was a pure cultivation of micrococci and produced fata^ 

 results in every case. The results of our inoculation experiments with 

 cultivated micrococci have heretofore been more or less unsatisfactory, 

 because, while the symptoms were those of s\a ine plague, the <lisease 

 produced did not correspond in its malignancy to the swine plague which 

 so frequently decimates the herds of the West. In this case, however, 

 the disease developing as a result of inoculation had all the malignancy 

 of the most severe outbreaks which I have ever witnessed, and in sub- 

 sequent experiments with virus obtained from these animals this fatal 

 type has been retained and every animal inoculated has succumbed. 



On July 3, pig No. 34 was inoculated with mixed pleural and perito- 

 neal effusion obtained from No. 28, which was killed that day in the 

 last stages of swine plague, produced by inoculation with cultivated 

 A'irus as detailed above. July 15, the temperature was 104° F., and 

 there were periods of shivering. From this time the progress of the 

 attack was rapid; there was a red blush of the skin over the abdomen, 

 diarrhea, loss of appetite, prostration, and tendency to hide in the litter- 

 July 18, it was very much debilitated, the breathing was rapid, and it 

 was scarcely able to walk. It would undonbtedl}^ have died in a few 

 hours. It was killed for examination and for pure virus. 



Auto])sy showed the lungs to be covered on pleural surface with [)ete- 

 chiic, but there was no hepatization. Tlie inguinal and mesenteric 

 glands were greatly enlarged and congested ; the small intestines in- 

 tlamed ; the ca'cum was the seat of three large ulcerous patches, 1 

 to 2 inches in diameter, and several of smaller size. These were black 



