ERADICATION OF PULLORUM DISEASE 1931-32 



35 



Table 12 — Data Concerning Exposures, Agglutination Titers, and 

 Necropsies for Pigeons in Group II 



* Agglutination not complete in any dilution. 

 ** Died. 

 *** Isolated from crop contents. 



The daily doses for the oral route group were extended over a period of 8 

 weeks. Bird 3 died during the eighth week about an hour after feeding. The 

 bird was bled at the time of feeding and no symptoms were observed. The cause 

 of death was not determined. S. pullorum was isolated from the ingluvies, but 

 not from the other organs. Birds 7 and 26 hatched 1 squab during the fifth 

 week and 2 during the ninth week. None of the squabs survived beyond 15 days 

 of age and 5. pullorum was not isolated on necropsy. Complete agglutination, 

 in any dilution, was not observed among the sera tested during the course of the 

 experiment. Birds 7, 20, and 26 were killed and necropsied 15 weeks after the 

 first exposure. No gross lesions were observed and 5. pullorum was not isolated. 



Birds 8, 12, 16, and 31 were given 0.5 cc. intraperitoneally for 3 successive 

 days. These doses were repeated after 7 daj^s. The birds were first tested on 

 the sixth day and no agglutination was observed. On the thirteenth day, com- 

 plete agglutination was produced by all sera except one, that of bird 12. This 

 bird's maximum titer was attained during the fifth week. After the titers of all 

 the birds had reached the maximum, a marked and rapid decline was observed. 

 The birds were killed and necropsied 15 weeks after the first exposure. Adhesions 

 of the peritoneum and a ruptured yolk were found in bird 12. 5. pullorum was 

 isolated from the yolk material. Complete agglutination was not produced by 

 serum of this bird at necropsy. 5. pullorum was not isolated from birds 8, 16, 

 and 31. 



The 2 birds exposed by ocular instillation received 46 daily doses (0.04 cc.) 

 extending over a period of 8 weeks. The agglutinin response was slight in bird 

 10 and negative in bird 28. The birds were killed and necropsied 15 weeks after 

 the first exposure. No gross lesions were observed and S. pullorum was not iso- 

 lated. 



The 2 control pigeons remained negative to the agglutination test during the 

 course of the e.xperiment. They were not killed. 



Group III, 5 pigeons, was placed in contact with pullorum diseased adult 

 chickens in an 8 x 12 foot house provided with a sun porch (8 x 12 feet). The 

 number of pullorum diseased chickens varied from 10 to 25 during the course of 

 the experiment. Nest boxes, for use of the pigeons, were fastened to the walls. 

 Feed and water were provided in common with the chickens. Mash was placed 

 in hoppers and scratch grain fed in the litter. The pigeons were tested at 4-week 



