ERADICATION OF PULLORUM DISEASE 1931-32 61 



favorable. On December 12, the flock owner learned that 22 birds had escaped 

 the test on December 1. These birds were immediately tested by both methods. 

 All the birds on the premises were confined in three different houses, which 

 necessitated moving the equipment three times. The time required to test all 

 the birds was approximately 5H hours. 



The results of the first test were as follows: 



Whole Blood Tube 



Test Test 



Number of birds positive 23 27 



Number of birds doubtful 25 



Number of birds negative 194 214 



Retest with W.B.T. 



* only, on Dec. 4 



Number 



Group Classification of Birds N D P 



I Positive to both tests 17 



II Negative to both tests 191* 



III Positive to W.B.T. and negative to tube test 6 4 11 



IV Doubtful to the W.B.T. and negative to tube test.. 17 17 

 V Doubtful to the W.B.T. and positive to tube test.... S** 12 3 



VI Negative to the W.B.T. and positive to tube test.... 2 10 1 



* Does not include the bird that reacted negatively to W.B.T. and whose blood sample was 



broken. 

 ** Two birds were not retested with the whole blood test. 



Based on the whole blood retest, the two birds which were negative to the 

 tube test and doubtful and positive respectively to the whole blood test, and 

 the two birds which were negative to the whole blood test and positive to the 

 tube test, were submitted to the laboratory for necropsy. The necropsy results 

 of these four birds and of two birds included in the test of December 12 are as 

 follows: 



Testing Results at Necropsy Necropsy Remarks 



Tube Test 



-5. pullorum 



Group No. Necropsy W.B.T. 10 20 40 80 160 320 640 Isolated Source 



f 11780 

 < 11756* 

 [ 11731* 



1/21 P? 2 10 



1/18 N 



1/21 P? 4 4 3 3 10 



1/22 P 4 4 4 2 2 



1/22 P 4 4 4 2 2 1 



Cyst and ovary 



* These two birds which reacted doubtfully to the W.B.T. and positively to the tube test on 

 December 12, were not retested with the W.B.T. 



On December 30, the second test, 224 birds were tested by both methods. 

 The technique of the whole blood method, as employed on December 1, was 

 modified. The test fluid was placed on the glass plate with a medicine dropper 

 held in a vertical position. One drop was equal to 0.05 cc. The blood was added 

 to the drop of test fluid with a wire loop (5 mm. in diameter). A loopful of blood 



