ERADICATION OF PULLORUM DISEASE 1931-32 



63 



Necropsy 

 Testing Results at Necropsy Remarks 



Flock II revealed 8.26 per cent reactors as determined by the agglutination 

 test. This flock had not been tested previously. The reactors were distributed 

 throughout the entire flock. 



AH the birds on the premises were tested 3 times at 4- to 6-week intervals. 

 On January 7, 8, and 9, a total of 691 birds was tested by both methods. The 

 testing operations and technique on the first two days were the same as employed 

 in the second test of Flock I. On January 9, only one assistant was furnished 

 which necessitated modifying the procedure in order to conserve time. The per- 

 son conducting the whole blood test did not bleed birds, but instead obtained 

 blood from the incision made by the person collecting samples for the tube test. 

 The mean temperatures for the three days were 40.5°, 30.5°, and 32.5° F., 

 respectively. The time required to test the entire flock with the whole blood 

 test was 15 hours and 45 minutes. The results of the tests were as follows: 



Whole Blood Tube 



Test Test 



Number of birds positive 39 48 



Number of birds doubtful 5 13 



Number of birds negative 647 630 



Group Classification Number of Birds 



I Positive to both methods 37 



II Negative to both methods 630 



III Doubtful to W.B.T. and positive to tube test 4 



IV Doubtful to W.B.T. and doubtful to tube test 1 

 V Negative to W.B.T. and positive to tube test 7 



VI Positive to W.B.T. and doubtful to tube test 2 

 VII Negative to W.B.T. and doubtful to tube test 10 



The two tests did not agree on 24 birds. These birds, included in Groups 

 III to VII, inclusive, were retested on January 14. The results are given in Table 

 25. 



The birds were classified into three groups, according to the results of the 

 retest. Four birds reacted positively with both methods. These birds were dis- 

 posed of with the remainder of the positive reactors. Among the remainder of 



