ERADICATION OF PULLORUM DISEASE 1931-32 



65 



the 24 birds, 10 were retained in isolation on the premises and 9 were submitted 

 to the laboratory for necropsy. Two birds were not necropsied: one was de- 

 stroyed by the flock owner and the other later reacted strongly positive to both 

 methods. The latter group was retested three times, including the test at the 

 time of necropsy. The results are given in Table 25. 



The birds in the retained group were retested on February 4 and 24. Follow- 

 ing the test of February 24, this group was submitted to the laboratory for ne- 

 cropsy. The testing results and the findings at necropsy are shown in Table 25. 



On February 4 and 5, Flock II was retested by both tests. The technique of 

 the whole blood test for February 4 was the same as that employed on January 

 7. On February 5, the technique of the whole blood test was slightly modified. 

 The blood collector who collected the samples for the tube test held the bird 

 for the tester of the whole blood test. After the tester had procured a loopful 

 of blood, the blood collector obtained a sample from the same incision. The 

 latter also placed the bird in the retaining crates. Two helpers, one catching 

 the birds and the other removing the tested birds from the retaining crates, 

 assisted in the testing. This change was made because the blood collector was 

 more familiar with the proper manner of holding the birds for bleeding. The 

 atmospheric temperature was approximately a few degrees above freezing. 

 The total number of birds tested with both methods was 610. The total time 

 consumed for the testing operations amounted to 12 hours and 10 minutes. The 

 results of the second test were as follows: 



* This table does not include the 10 reacting birds which were retained on the premises after the 

 first test. The testing results of these birds are reported in Table 25. The bird which was 

 positive to the whole blood test was also positive to the tube test. 



On March 23, Flock II was tested for the third time. The technique for the 

 whole blood test was similar to that of February 5, except that one assistant 

 recorded the leg band numbers and resultsf or the tester conducting the whole 

 blood test. The atmospheric temperature was slightly below freezing. A total 

 of 422 birds was tested in Q}4 hours. The results of the tests were as follows: 



Whole Blood Tube 



Test Test 



Number of birds positive 



Number of birds doubtful 3 2 



Number of birds negative 419 420 



These five doubtful reacting birds were submitted to the laboratory for 

 necropsy and the results were as follows: 



Group 



Bird 



No. 



Results of Necropsy 



Tube S. pullorur, 



Test Isolated 



f 75182 



Doubtful to W.B.T. \ 80271 



i 80543 



Doubtful to tube test <! §0124 



3/30 

 3/30 

 3/30 



3/30 

 3/30 



