Testing Plan No. 1. — Tests on Birds One Year of Age or Older. 

 A flock of hens is to be considered free from infection when reactors do not exist in the breeding 

 flock after the following plan has been carried out: — 



(a) First test at the age of one year or older. 



(b) Second test on non-reactors, six to twelve months later. 



By this plan, each adult in the breeding flock will have been tested twice. 



Testing Plan No. 2. — Tests on Pullets. 

 A flock of pullets is to be considered free from infeclion when it is the product of a flock which 

 has already been tested twice, and when on test it contains no reactors. Three tests are thus 

 required, as follows: — 



(a) Original test when the birds are eight to twelve months old. 



(b) Non-reactors tested again six to twelve months later. 



(c) Third test made on the offspring of the birds already tested twice. 



Testing Plan No. 3. — Alternate Plan for Tests on Pullets. 

 A flock of pullets is to be considered free from infection when produced as per the following 

 plan, and when, on the last of the following tests, no reactors are found to exist. The procedure 

 is as follows: — 



(a) Original test when birds are eight to twelve months old. 



(b) Non-reactors tested again four to six weeks later. 



(c) Offspring of the above flock tested as pullets. 



(d) Non-reactors tested again four to six weeks later. 



In other words, the test is applied to pullets and their offspring. By this plan each bird is 

 tested twice before being bred. 



Note. — It is mutually understood that pullets are eligible for testing two months after the 

 flock has attained a 20 per cent egg production. Male birds may be used for breeders if tested 

 once and found non-reacting. 



Control Measures. 



A. All breeding birds, male or female, to be leg-banded with bands furnished at cost by the 

 Department of Veterinary Science and Animal Pathology of the Massachusetts Agricultural 

 Experiment Station. 



B. All birds showing a positive agglutination reaction to be removed from the breeding 

 flock. Reacting birds to be disposed of if possible ; but if it is foimd necessary to keep them as 

 egg producers, they are to be kept under strict quarantine. 



C. Male birds not to be housed during the breeding season with other than breeding stock. 



D. New stock brought on the place, whether adults, day-old chicks, or hatching eggs, to 

 come from stock shown to be free from bacillary white diarrhoea, as determined by the aggluti- 

 nation test. 



Note. — The Department of Veterinary Science and Animal Pathology of the Massachusetts 

 Agricultural Experiment Station does not issue certificates of any kind or enter any agreement to 

 guarantee flocks. It will, however, co-operate with poultry associations or groups of poultrymen 

 who may wish to issue certificates of merit or accredit their own flocks. 



