10 LABORATORY EXERCISES IN BACTERIOLOGY. 



methods, and at the close of the book a tabulated blank is inserted, following which the 

 preservation of the notes of observation in such work should be made. 



Laboratory Cleanliness. In the pursuit of such individual practice it is scarcely 

 necessary to insist upon the utmost care and cleanliness on the part of each worker at all 

 stages of the work. All appliances are to be kept in constant order and cleanliness, and 

 whenever exposed to contamination in any manner should be subjected to thorough ster- 

 ilization in the most suitable manner. Not only this, but the individual who is person- 

 ally scrupulously clean is not only the most successful worker, but more certainly than 

 the careless escapes the possible danger of infection. Suitable receptacles are provided 

 for containing discarded material in disinfectant solutions, and such substances are 

 later burned ; and each student should bear in mind as a constant duty to see that all 

 such matter is promptly and properly rendered harmless. Personal habits must in- 

 variably give way, in all the manipulations of the laboratory, to the precise care of 

 surgical procedures on the living subject ; and it is desirable that even in the matter of 

 clothing similar precautions toward cleanliness and protection should obtain. 



In case of accidental breakage or spilling of a tube containing a culture upon the 

 floor, table, or elsewhere, as much as possible should be wiped up with a wet rag at once 

 and the rag thrown into the laboratory disinfecting jar, to be burned later ; and a strong 

 disinfectant solution should be freely poured over the contaminated surface and allowed 

 to remain for a half hour, after which it may be wiped up with a mop and the mop de- 

 stroyed. Clothing thus contaminated should be boiled or baked ; and the hands should 

 be thoroughly disinfected and washed well with soap and water. 



Laboratory Provisions. Each student is furnished with a suitable work-table, with 

 non-absorbent top, provided with water and gas connections ; for general use are pro- 

 vided the various types of sterilizing apparatus, incubator space, serum 'inspissators, 

 gas generators, material for culture media, chemical tests, staining agents, scales, inocu- 

 lating apparatus, and animals for inoculation. Each student is provided with an indi- 

 vidual locker containing the following pieces of apparatus, .and can obtain by applica- 

 tion the less frequently needed pieces for special work: 



1 microscope with usual accessories, with high-power objective and bottle of im- 

 mersion oil, and with micrometer ocular. 



6 dozen culture test-tubes. 



1 dozen large test-tubes for potato cultures. 



1 large double dish for potato and plate cultures. 



10 small double dishes (Petri dishes). 



1 straight platinum needle with glass handle. 



1 looped platinum needle with glass handle. 



Assorted glass rods and glass tubing. 



1 50 c.c. pipette. 



1 5 c.c. pipette. 



2 glass beakers. 



2 500 c.c. Erlenmeyer flasks. 



1 small glass funnel. 



1 large glass or granite funnel. 



1 large granite pan. 



1 granite water-bath pan (bain marie). 



1 granite dipper, 500 c.c. capacity. 



1 filtration bucket. 



1 gas stove. 



