viii TABLE OF CONTENTS. 



LESSON VI. PAGE . 



GROSS APPEARANCE OF BACTERIAL CULTURES, 172-184 



Colonies of Bacteria, 172; Observed Features, 172; List of Cultural 

 Characters, 174. 



LESSON VII. 



INDIVIDUAL BACTERIA, THEIR PHYSICAL AND CHEMICAL CHARACTERISTICS, . . . 186-244 

 Microscope, 186; Slides and Cover-glasses, 188; Microscopic Examina- 

 tion of Colonies, 188; Hanging-drop Preparations, 188; Film Prepara- 

 tions, 192; Staining Reagents and Mixtures, 194; Staining Methods, 202. 

 Physical Characteristics of Bacteria: Shape, 208 ; Grouping, 210 ; Structure, 

 Capsules, Flagella, Nuclear Granules, 212; Size, 216; Motility, 218; Re- 

 production, 220; Formation and Germination of Spores, 222; Determi- 

 nation of Spores, 224. Chemical Activities of Bacteria: Pigment Forma- 

 tion, 226 ; Photogenic Power, 226 ; Ferments : Proteolytic Ferments, 228 ; 

 Diastasic Ferments, 230; Invertin Ferments, 230; Rennet Ferments, 

 230 ; Gas Production, 232 ; Acid Production, 234 ; Alkaline Production, 

 234; Alkaloidal Products, 236; Toxalbumins, 236; Antitoxins, 236; 

 Agglutination Phenomenon, 238; Indol and Phenol Production, 240; 

 Nitrifying and Denitrifying Bacteria, 242. 



LESSON VIII. 



ISOLATION OF BACTERIA IN PURE CULTURE, 246-260 



Mechanical Methods: Plates, 246; Petri Dishes, 246; Esmarch Tubes, 

 246; Salomonsen's Capillary Tubes, 248: Klebs' Fractional Method, 252. 

 Physiologic Methods: Selection by Living Animal Tissues, 254; Selection 

 by Culture Media and Modifications, 256 ; Selection by Culture Tempera- 

 ture, 258; Selection by Culture Atmosphere, 258; Cohn's Heating 

 Method, 260. 



LESSON IX. 



CLASSIFICATION AND IDENTIFICATION OF BACTERIA, 262-274 



Classification, 262 ; Table of Classification into Genera, 264 ; Analytic 

 Table, 268 ; List of Equivalent Nomenclature of Genera, 272. 



LESSON X. 



PATHOGENIC ACTION OF BACTERIA, 276-290 



Importance of Study, 276 ; Selection of Experiment Animals, 278 ; Hypo- 

 dermic Inoculation of Animals, 278; Intravenous Inoculation, 280; In- 

 traperitoneal Inoculation, 282; Other Forms of Inoculation, 282; Care 

 and Observation of Experiment Animals after Inoculation, 284 ; Autopsy, 

 286; Immunity, 288. 



BACTERIOLOGIC CHART. 



INDEX, . 291 



