TABLE OF CONTENTS. 



LESSON I. PACK. 



INTRODUCTORY, 9-14 



Plan of Work, 9; Laboratory Cleanliness, 10; Laboratory Provisions, 10 

 Place of Bacteria in Nature, 12; Preliminary Classification, 12; Impor- 

 tance to Man, 14. 



LESSON II. 



STERILIZATION, 16-56 



Occurrence of Bacteria in Nature, 16. Sterilization: Flaming, 18; Dry- 

 air Oven, 20 ; Boiling, 24 ; Steam Sterilization by Ordinary Method, by 

 Autoclave, and by Pasteurization, 24 ; Determination of Thermal Death- 

 point, 32; Sterilization by Filtration, 36; Sterilization by Chemical 

 Solutions, 40 ; Determination of Disinfectant and Antiseptic Values, 46 ; 

 Disinfectant Gases, 54. 



LESSON III. 



PREPARATION OF TUBES, FLASKS, DISHES, ETC., FOR CULTURE MEDIA, 58-68 



Tubes, 58; Cleansing Tubes, 58; Plugging Tubes, 60; Sterilization of 

 Tubes, 62; Special Forms, 62; Flasks: Uses, Forms, Cleansing, etc., 64. 

 Dishes : Culture Dishes, Their Preparation and Uses, 64 ; Petri Dishes, 66 ; 

 Plates, 68. 



LESSON IV. 



CULTURE MEDIA, 70-106 



Purposes and Character, 70; Reaction, 70. Carbohydrate Media: Prepara- 

 tion of Potatoes for Dish Culture, 72 ; for Tube Culture, 76 ; Glycerine 

 Potato, 78; Eisner's Medium, 78; Holz's Potato Gelatine, 78; Other 

 Carbohydrate Media, 78. Proteid Media: Bouillon, 80; Sugar-contain- 

 ing Bouillon, 82; Distribution of Bouillon and Other Liquid Media to 

 Tubes, etc., 84; Gelatine, 86; Agar, 90; Gelatine-agar, 92; Glycerine 

 Agar, 92 ; Lactose-litmus-agar, 94 ; Peptone Solution, 94 ; Rosolic Acid- 

 peptone Solution, 94; Blood-serum, 94; Loeffler's Medium, 104; Milk, 

 104; Litmus Milk, 106. Preservation of Media, 106. 



LESSON V. 



INOCULATION OF MEDIA AND CULTIVATION OF BACTERIA, 108-170 



Appliances for Inoculating: Platinum Needle, 108; Swabs, 112; Pipettes, 

 114; Syringes, 116; Sternberg's Bulbs, 116; Blades, 118; Forceps, 118; 

 Particles, 118; Fractional Inoculations, 120. Source and Collection of 

 Material for Inoculation, 122. Cultures: Tube Cultures, 124; Plate Cul- 

 tures, 124; Dish Cultures, 126; Esmarch Tubes, 128. Illustrative Proce- 

 dures: Examination of Air, 128; Examination of Water, 134; Examina- 

 tion of Milk, 142; Examination of Soil, 144; Examination of Material 

 from Autopsy, 144. Cultivation of Inoculated Material: Conditions of 

 Bacterial Growth, Selection of Nutrient, 148; Moisture, 148; Tempera- 

 ture, 150; Incubator, 150; Thermostat, 152; Gas-pressure Regulator, 

 156; Atmosphere, 156; Anaerobic Jar, 160; Anaerobic Tubes, 160. 

 Numerical Estimation of Bacteria, 162. 



