8 



it would have fruited if the skin had not 

 dried up. 



Dr. Halsted reports having been suc- 

 cessful in inoculating the lemon with G. 

 fructigenum. 



4. BANANA inoculated from Gloeosporium 

 of the Banana. The Gloeosporium began 

 to fructify on the seventh day, before any 

 of the other fruits that were inoculated at 

 the same time with the same material had 

 begun to show signs of growth. This may 

 tend to show that a Gloeosporium which 

 has been produced on a given fruit acts 

 more promptly on that fruit than on others 



Fig. 8. Spores from an old 

 pustule of the Gloeosporium 

 found growing naturally on 

 the banana. 



X 400 



of a different kind. Or it may be that the 

 difference in the time of germination in this 

 case was due to the fact that the fungus 

 having just completed a cycle in the banana 

 found itself again on ground of precisely 

 the same stage of ripeness. In other 



Fig. 9. Gloeosporium spores, 

 obtained by inoculating a 

 banana from a banana. 



words, the slower growth on the other 

 fruits soon to be mentioned may have been 

 an effect not of the change in the host, but 

 in the condition of the host. 



On the twelfth day the banana was 

 thoroughly infested. (Figs. 8 and 9. ) 



5. BANANA inoculated with the Gloeo- 

 sporium of the Banana after the fungus 

 had been passed through the Quince. 

 Several bananas were inoculated. All 

 promptly showed signs of having taken, 

 and all became infested. 



6. GRAPE inoculated from Gloeosporium 

 of the Banana. At the end of thirteen 

 days the grape showed traces of one or 



Fig. 10. Spores of the Gloeos- 

 porium, obtained by inocu- 

 lating a grape with spores 

 of the Gloeosporium found 

 growing naturally on the 



two dark unruptured pustules of con- 

 siderable size. The result on the grape, 

 which was of the large white firm-fleshed 

 long-keeping type, was in striking contrast 

 with the considerable number of other 



inoculations made from the same source. 

 The pustules were few and dark, and the- 

 growth was slow. There was a con- 

 centric and dark appearance reminiscent 

 of the Black Spot or Anthracnose of the 

 Grape. 



On the fourteenth day pustules were 

 removed from under the skin of the grape- 

 and examined. The mycelium resembled 

 that of a Gloeosporium, and spores measur- 

 ing 5-7 x 7-14/i were found, coloured as in 

 the banana. Thus it was shown that these- 

 spores were smaller and rounder than on 

 the banana, though some of them reached 

 the size 6 x 14]u. (See Fig 10.) The 

 colour and structure were identical with 

 those of the original spores. I never saw 

 any of the pustules on the grape burst 

 open promptly and emit spores freely as 

 was usually the case on other fruits. It 

 was always necessary at this stage, in 

 order to secure spores for examination, in 

 the case at any rate of this particular 

 variety of grape, to dig beneath the skin 

 and remove the lenticular- shaped spore- 

 beds. 



On the twenty-third day the pustules 

 burst, and it was interesting to note that 

 the spores were of a little brighter green 

 colour than other spores resulting on other 

 fruits in the same series of inoculations, 

 and that they had, perhaps, a little coarser 

 granulation. There were also some irregu- 

 larities in the form, some spores being more 

 pointed at one end than the other. 



7. PEAR inoculated from Gloeosporium 

 of the Banana. At the end of the- 

 twelfth day the pear gave slight evidence 

 of infestation. 



At the end of sixteen days great numbers; 

 of pustules were to be seen on the pear, 

 but none of them had broken through the 

 epidermis. Most of them were dark- 

 coloured, a feature of these pustules in- 

 certain states and varieties of the pear. 



On the twenty-first day it was found that 

 the dark-coloured pustules which were still 

 under the skin of the pear bore ellipsoidal, but 

 not yet mature, spores. The dark colour of 

 the pustules was due for the most part to the 

 colour of the fungus elements themselves, 

 and not to the pigment of the skin of the 

 pear. No doubt there was a discolouration 

 of the skin of the pear through the action 

 of the fungus, but this was not the main 

 element in the dark colour of the pustules. 



There is a most decided difference in the 

 appearance of the fructification of Gloeo- 

 sporium in pears of different varieties. 

 According as the pear has been evolved for 

 earliness or lateness, for immediate con- 

 sumption or for long keeping, for sweetness- 

 or for acidity, for mildness or for astrin- 

 gency, there have been produced in the 

 fruit properties having, it appears, a 

 decided influence on the history of its 

 Gloeosporium parasite. 



