9 



8. PASSION-FRUIT inoculated from the 

 Banana Gloeosporium. The inoculation 

 took, but after a month no pustules had 

 formed. Concentric with the inoculation 

 point was an area about an inch in diameter, 

 which had taken on a reddish-purple colour. 

 This was the evidence upon which the 

 Gloeosporium was believed to have taken. 

 It is known that a species of Gloeosporium 

 occurs in nature on the passion-fruit, so 

 similar in its spore characteristics to the 

 Gloeosporium of the pomaceous fruits that 

 it is difficult to point out any difference. 

 I have myself seen and carefully examined 

 this Gloeosporium of the passion-fruit. 



9. DATE-PLUM inoculated from Banana 

 (see Figs. 11 and 12). After twelve days 

 the date-plum showed slight evidences 

 of being diseased at the point of inocu- 

 lation. On the next day pustules were 



Fig- 11. Spores of the Gloeo- 

 sporium obtained by inocu- 

 lating the date-plum with 

 spores of the Gloeosporium 

 found growing naturally on 

 the banana. Compare with 

 Fig. 12. 



noted under the skin of the date-plum. 

 They appeared to be finer and closer 

 together than on the other fruits inocu- 

 lated from the same source. It seemed 

 as if the fructification met some resist- 

 ance from the skin of the date-plum, which 

 is known to be long resistant to decay. 

 Probably the skin of this fruit, notwith- 

 standing its thinness, is rather more re- 

 sistant than that of other fruits. I think 

 the keeping qualities of the date-plum bear 

 out this theory. If they are not knocked 

 about these fruits keep for a long time 

 without decaying, although the pulp may 

 meanwhile become remarkably soft and of 

 such a nature that it would most readily 

 decay if once yeast fungi or bacteria should 

 gain entrance. It may also be that the 

 astringent property of the date-plum has 



Fig. 12. Spores from inocula- 

 tion on date-plum, secured 

 by inoculating with spores 

 from the Gloeosporium 

 found growing naturally on 

 the banana. These spores 

 are from an old pustule, 

 and should be compared 

 with those from a mature 

 but younger pustule, shown 

 in Fig. 11. 



X400 



something to do with the difference in 

 the appearance of the Gloeosporium pustules 

 on this fruit. ' On the fourteenth day pus- 

 tules were removed from under the skin 

 and spores were found to exist in them in 

 abundance, and they were identical in form, 

 size, and colour with those of the banana 

 and quince of the same series of inocula- 

 tions. The spores measured 5-6 x 12- 14/u. 



Still, for a long time afterwards the 

 pustules remained sub-epidermal and the 

 spores continued to show irregularities due 

 to resistance of the skin to rupture. 



From the somewhat exceptional action 

 of the Banana Gloeosporium on the date- 

 plum it might be thought that the con- 

 ditions were uncongenial. Thus the pus- 

 tules were smaller than usual in other 

 members of this series of inoculations, and 

 the spores were tardy in their outbreak, as 

 has been already mentioned. 



Nevertheless, when spores derived from 

 this date-plum were used to inoculate the 

 banana once more, they produced the cha- 

 racteristic disease with promptitude. In a 

 week's time diseased areas had developed 

 bearing scores of well-grown pustules, whose 

 spores were of the form, size, and colour 

 characteristic of the Banana Gloeosporium, 

 in spite of the fact that the " seed " taken 

 from the date-plum were undersized and 

 distorted. 



10. PEAR inoculated from Gloeosporium 

 of the Banana. After twenty-three days 



(ff^) |p Fig. 13. Spores of the Gloeo- 

 sporium from inoculation 

 O^ on pear from spores found 

 pi) growing naturally on th& 

 ml banana. 



* 400 



the rotten area on the pear was 1 inches' 

 across, and bore hundreds of pustules, from 

 some of which spores were being emitted 1 

 in " tendrils." 



11. QUINCE inoculated from Gloeosporium 

 of the Banana. Not successful. The in- 

 oculation seemed to take in a partial 

 manner. After twenty-three days the spot 

 was only inch across, and was but little 

 sunken. It was mottled brown in colour 

 and dry looking, with no indications of 

 pustules. It seemed as if the quince was 

 not in the right condition for receiving the 

 fungus perhaps too green. 



12. QUINCE inoculated from Gloeosporium 

 of the Banana. At the end of twelve days 

 the rotten area was upwards of half an 

 inch across, and bore fruiting pustules, 

 often dark coloured, from which salmon- 

 coloured spores were oozing. There was 

 an appearance of the concentric rings- 



Fig. 14. Spores of Gloeospor- 

 ium, obtained by inoculating 

 the quince with spores found 

 growing naturally on tbe- 

 banana. 



characteristic of the ripe rot of the apple. 

 This is the quince from which spores were 

 used to reinoculate a banana (No. 5) ; the 



