10 



inoculation on the banana was entirely 

 successful. 



On the thirteenth day the spores from 

 this quince were compared with those from 

 a banana that had been inoculated from the 

 same source, namely, a banana on which 

 the Oloeosporium was found growing in 

 nature. The spores on the quince were 

 12-18 x 6/j., and were of practically the 

 same dimensions as those of the inoculated 

 banana, 11-14 x 6-7/*, but they had a 

 coarser internal granulation and more of a 

 tendency to be pointed at one end. The 

 colouration in the two sets of spores was 

 identical. 



13. APPLE inoculated from Gloeosporium 

 of the Banana. No resulting disease. 

 The apple was a tough -looking Russet. 

 This experiment was afterward repeated 

 with the result of verifying the records of 

 others that the disease can be easily trans- 



X 400 



Fig. 15. Spores of Gloeosporium, grown on the 

 apple from an inoculation from banana Gloeos- 

 porium. These spores are somewhat unripe, 

 i.e., were taken from pustules of slow growth, 

 and not yet burst. 



ferred to the apple. The nature of the 

 apple utilised in the first trial explains the 

 failure of the inoculation, and emphasises 

 the fact of specific resistance. 



14. PEACH inoculated from Gloeosporium 

 of the Banana. For a long time it appeared 

 as if this inoculation was a failure, but 

 ultimately a virulent outbreak of spores 

 occurred. On the seventeenth day the 

 peach was examined for the first time in 



Fig. 16. Gloeosppnuin spores, 

 obtained by inoculating a 

 peach with spores from the 

 Gloeosporium found grow- 

 ing naturally on the banana. 



X400 



several days, and it was found to be almost 

 literally covered with deliquescent spore 

 masses of a salmon colour (Fig. 16). 



The skin of the peach is such that the 

 early stages of the growth are not very 

 apparent 



The spores measured 6-7 x 20-25/i, these 

 being the largest secured on any fruit in 

 this particular series of inoculations (in- 

 oculations from banana on to nine different 

 fruits belonging to various orders). 



Spores taken from this peach reproduced 

 the disease on the banana. It was found 

 to be impossible to measure the size of the 

 pustules on the peach. Clavate spores of 

 somewhat greater length were seen, 27 /u. 



15. QUINCE inoculated from Gloeosporium 

 of the Guava. Took readily, the fungus 

 coming into fruit after five days, and con- 

 tinuing to fruit abundantly thereafter. 

 The spores (see Fig. 17) showed a varia- 

 tion from the original form as found on the 

 guava, the variation being in the direction 

 of the form found on the pomaceous fruits. 



Fig. 17. Gloeosporium spores, 

 obtained from an inocula- 

 tion on quince with spores 

 from the Gloeospomim 

 found growing raturally on 

 the guava. 



x 4oo 



Spores from this culture were used to 

 inoculate a guava, and with success. (See 

 Inoculation 19.) 



16. HAWTHORNE BERRIES were inoculated 

 with the Gloeosporium of the Guava after 

 it had been passed through the apple. 

 The berries were of Crataegus oxyacantha, 

 and were gathered in June near Sydney, 

 being fully ripe at the time of gathering. 

 After nine days, pustules had formed and 

 burst open, though the spores of the 



Fig. 18. Spores of the Gloeo- 

 sporium found growing 

 naturally on the fruit of the 

 guava. 



X400 



Gloeosporium had not yet formed. The 

 spore beds of mycelium were fully formed, 

 and, as usual, consisted of closely-packed 

 septate threads, at the summits of which, 

 however, there was, as yet, no indication 

 of the formation of spores. 



On the eleventh day well-formed spores 

 were secured and drawn. In form $nd 

 size (4'2-5'6 x 9-21/t, and averaging 

 14 x 4'8/x) they resembled closely the 

 original spores as found growing in nature 

 on the guava. (See Fig. 18.) 



16a. APPLE inoculated from Gloeosporium 

 of the Guava. Took rather slowly, the first 



Fig. 19. Spores of the Gloeos- 

 porium, produced on apple 

 b.T inoculating it with Gloeos- 

 porium spores found growing 

 naturally on he guava. 



X 400 



spores being obtained after ten days. 

 There was a manifest difference between 



