13 



a punctate appearance. The number of spores in each pustule was 

 not great, but the spores were of large size, measuring 6- 5-8 x 18-32/x. 

 In form they were, for the most part, elongated, 

 but in some instances they were somewhat 

 fusiform (Fig. 28). The colour was nearly that 

 of the Gloeosporium of the guava, but it was a 

 trifle less brilliant, the iridescence being a little 

 less marked. The vacuole characteristic of most 

 spores of Gloeosporium was not plainly to be 

 seen > the ? ne granulation being such as to 

 obscure it, if it existed at all. 

 It will be seen that the spores of this form are larger than any 

 others examined in the course of these experiments. 



Although the spores when freshly removed from the pustules appeared 

 to be one-celled, after twenty-four hours in water they were multi- 

 cellular, some two-celled, some three-celled, some four-celled, the 

 latter being more constricted at the middle division. After the 



Fig. 29. Spores of the Gloeosporium, <3H3& /& Fig. 30. Gloeosporium, found 



found growing naturally on the growing on the fruit of 



berries of the Hawthorne. These <J^^> M Hawthorne (Crataegiis) , 



are shown in the first stages of June, 1902, at Killara, 



germination. It is not often that New South Wales, 



the spores become four-parted in x 4Q _ r 

 this manner before throwing out 

 a definite mycelial thread. 



formation of these septa the vacuoles in the various cells appeared a 

 trifle more discernible (see Fig. 29). The cells germinated vigorously, 

 sending forth a mycelium that at the end of twenty-four hours was 

 many times as long as the spore, and considerably branched, but 

 with no tendency to produce conidia. 



Ripe-rot fungus on leaves of Passion-vine. (Fig. 31.) 



The Gloeosporium found on the leaves of the passion- vine was used 

 to inoculate a pear, a banana, two apples, and a'passion-fruit. After 

 thirty days, the pear, which had become slowly infected from the first, 

 was found overrun with Penicillium, in the midst of which, at least 

 on the borders of the outbreak, pustules of Gloeosporium were to be 

 found. These gave spores somewhat smaller than those of the pustules 

 on the passion-vine leaves from which they were inoculated. The 

 measurements are given herewith. 



It will be seen that the average dimensions are 6'2/* x 



13-6 x 6-e 13 ' 4 > the limits bein g 5 ~ 9 x 12-15/a. 

 12-6 x 5-2 M As many of the spores had germinated, it is not 



?. 7 x ?! M possible to give these measurements the weight they 



13 '3 X O2U 



15 -3 x 7-0 M 



11 '9 x 8'7 U ( [ ^Ms* 31. Gloeosporium of the leaves of the 



passion-vine, germinating and producing 



secondary spores. 



13-4x6-2^ 



would otherwise have. The spores that had not 

 germinated seemed to me to be dwarf spores, and those that had 



