SYMPTOMS AND PATHOLOGY 



all Others, whose number is considerable, I obtained, 

 by the culture test in nutrient gelatine, evidence of the 

 presence in the lungs and the liver of numbers of 

 bacteria belonging to one and the same species ; for qr(: ^ 

 when a particle of the lung or liver tissue is rubbed over 

 the slanting surface of nutrient gelatine, or when it is 

 shaken up in a test-tube with melted sterile nutrient 

 gelatine, and the latter is then poured into a sterile 

 glass dish, allowed to set and then incubated — that is 

 if a plate cultivation in gelatine is made — colonies of 

 one single bacterial species are obtained. By making 

 sections through the inflamed lung or liver, after har- 

 dening, and staining these sections for several hours 

 in methyl blue, or better still in rubin and methyl 

 blue, it is found that in some parts some of the 

 capillary blood-vessels, both in the lung and in the 

 liver, contain continuous masses, plugs or emboli, of 

 the same bacterial species (Figs. 2, 3, 4, 15 and 16).^ 

 But there are seen under the microscope extensive 

 parts in which the blood-vessels do not contain them. 

 It is from this easy to see why I failed in 1887 to 

 obtain the microbe from the lung or liver in cover- 

 glass specimens or in culture, viz. I used in both 

 cases a droplet of the blood of the lung or liver, 



1 In sections through the hardened liver stained with rubin, then 

 with blue, the clumps of microbes are brought out with great dis- 

 tinctness as blue masses on a red ground. 



