IV CULTURE BY SUBCUTANEOUS INOCULATION 41 



and the heart's blood ; in the latter, however, they are 

 not numerous ; in some cases they could not be found 

 in the blood, though they were present in the inflamed 

 lungs. 



I have further tested, as regards virulence, the 

 sub-cultures derived from the blood and lung of the 

 mouse, and from the blood and lung of the ammer, 

 on mice, guinea-pigs, and ammers, and have found 

 that there is no difference between them and the sub- 

 cultures obtained from the lung of the grouse. While, 

 then, by these experiments it follows that the cultures 

 of the microbe act virulently when subcutaneously 

 injected into mice, guinea-pigs, buntings, ammers, 

 and sparrows, they do not produce fatal infection in 

 rabbits, and do not produce any result in pigeons or 

 fowls ; and for these reasons alone the microbe as 

 well as the disease it produces is well defined from 

 chicken cholera, which, as we shall show later on, 

 is very characteristic as regards the virulence of its 

 specific microbe on rabbits, fowls, and pigeons. As 

 we shall also show, there exists a certain similarity 

 in shape, in certain cultural characters, and in the 

 non- liquefaction of the nutrient gelatine, between the 

 microbe of grouse disease that we have been hitherto 

 considering, and the microbe of chicken cholera ; but 

 the differences in the growth of the two microbes in 

 the various culture media are sufficiently striking 



