FOJVL CHOLERA 85 



assuming the bacilli to be distributed throughout the 

 blood in a uniform manner, it was calculated that, 

 provided the relation of blood to body weight be 

 about I to 14 or 15, there were present in the total 

 blood over 1 200 millions of bacilli, that is to say, that 

 the 20,000 bacilli introduced into the animal had 

 multiplied in 20 hours so as to yield 1200 millions, 

 or in other words, that each one of the introduced 

 bacilli had produced a crop of 60,000 bacilli. 



(2.) On examining sections of the hardened liver of 

 animals — fowl, pigeon, or rabbit, — dead of the disease, 

 there are always found in each section patches of the 

 liver acini in which the capillary vessels are distended 

 and filled with the bacilli ; the liver cells around being 

 dark, granular, and disintegrating ; in the fowl they 

 are filled with fat droplets, but in all parts of the 

 section all the blood-vessels contain crowds of the 

 bacilli, and many of them are present outside the 

 vessels between and around the liver cells (Figs. 

 38 and 39). 



The illustrations which are given in Figs. 32-40 

 give an accurate representation of the nature of the 

 gelatine cultivations, of the shape and size of the 

 microbe, and of their distribution in the blood and 

 the organs. 



