CHAPTER XI 



CULTIVATION OF THE BACILLUS OF FOWL ENTERITIS 



A DROPLET of blood taken from the heart and used 

 for a gelatine plate cultivation, or rubbed over the 

 slanting surface of gelatine, yields always a pure culti- 

 vation of colonies of the bacilli of fowl enteritis. The 

 number of colonies of a droplet of blood of a fowl dead 

 of fowl enteritis compared with the number of colonies 

 of a droplet of blood of a fowl dead of fowl cholera, as 

 may be expected from what has been said above, is 

 strikingly different. This difference can be made very 

 conspicuous by diluting the blood first and then mak- 

 ing cultivations. A measured drop of blood — one 

 minim or one division of a Pravaz syringe — of a fowl 

 dead of fowl cholera is added to lo cc. of sterilised 

 (boiled) salt solution, and well mixed ; from this a 

 droplet is taken by means of the platinum loop, and 

 with it a gelatine tube is inoculated, and from it a 

 plate cultivation is made ; or it is rubbed over the 

 surface of the slanting gelatine contained in a tube. 

 The number of colonies making their appearance in 



