XI CULTIVATION OF THE BACILLUS 95 



the second and third days, the line of inoculation 

 becomes visible as a greyish-white line, which under a 

 glass is seen to be made up of spherical dots ; on 

 further incubation these enlarge, but remain always 

 separated ; on the upper free end of the stab the 

 growth is a thin translucent greyish-white film with 

 crenate margin. 



The differences in the rapidity of the growth in 

 streak, stab, and shake culture shown by the bacillus 

 of fowl cholera and of fowl enteritis are very marked, 

 that of the latter being considerably more rapid than 

 that of the former. 



The gelatine is never liquefied by the growth. 



In faintly alkaline broth kept at 35-37° C. the 

 bacillus of fowl enteritis grows rapidly, forming 

 uniform turbidity in twenty-four hours ; the turbidity 

 gradually increases, and, compared with that produced 

 by the fowl -cholera bacillus, is always at the same 

 distance of time markedly greater ; there is also a 

 greater amount of whitish precipitate in the broth 

 cultures of the bacillus of fowl enteritis than in those 

 of the bacillus of fowl cholera ; there is never any 

 formation of anything like a pellicle. Broth cultures 

 of the bacillus of fowl enteritis kept at 35-37° C. 

 begin to get clearer in the superficial layers by the end 

 of the week. On nutrient Agar incubated at 35-37° 

 C. the bacillus of fowl enteritis forms a whitish-grey 



