98 THE GROUSE DISEASE chap. 



then incubated. Large numbers of colonies of the 

 bacillus of fowl enteritis make their appearance with 

 scarcely any other contaminating bacteria : I have in 

 this way obtained, directly from the intestinal mucus, 

 pure cultivations with numerous colonies on the slant- 

 ing surface of gelatine. 



Dr. F. H. Andrewes has in my laboratory ascer- 

 tained that various species of microbes, which, when 

 grown on the ordinary nutritive media, occur chiefly 

 as oval or short rod-like organisms, — of swine fever, 

 Middlesbrough pneumonia, grouse disease, — when 

 grown on similar media, but to which previously had 

 been added Stedman's sea salt, to the amount of five per 

 cent, always form filamentous and thread-like bacilli, 

 either straight or wavy, singly or apparently branched ; 

 and when cultivated back on the ordinary media 

 again produce growths of the oval or short rod-like 

 forms. Dr. Wood repeated for me these experiments 

 with reofard to the fowl cholera and fowl enteritis 

 bacilli, and he ascertained as a uniform result that 

 while the bacillus of fowl cholera does not grow in 

 the salted media (gelatine, Agar, beef broth), the 

 bacillus of fowl enteritis grows well therein, form- 

 ing peculiar curved filaments of various lengths, 

 sometimes very considerable, the threads being 

 single or apparently branched : but this latter may 

 be only apparently so, because a matting together 



