xii EXPERIMENTS ON FO WLS 103 



mortem examination the typical appearances were 

 found. The blood, spleen tissue, and intestinal mucus 

 contained the bacilli ; cover-glass specimens and culti- 

 vations proved that the intestinal mucus was almost 

 a pure culture of the bacilli of fowl enteritis. The 

 second fowl remained lively and showed no diarrhoea. 

 After the lapse of eighteen days since the feeding, 

 this fowl was inoculated subcutaneously with a particle 

 of a first sub-culture on gelatine. On the 5th day the 

 bird was quiet and had diarrhoea, on the 6th the 

 diarrhoea was profuse, and on the 7th the fowl 

 was found dead with the characteristic appearances 

 and the typical distribution in its body of the bacilli 

 of fowl enteritis. 



6. Two fowls were fed with a mixture, prepared 

 by mixing broth, Agar, and gelatine sub-cultures, the 

 two former about one month old, the last about six 

 weeks old. By the 7th day no sign of illness was 

 noticed in these fowls. They were fed again with a 

 gelatine sub-culture, eleven days old, but no illness 

 was noticed by the 8th day. 



From this series it follows that by feeding with 

 the intestinal contents the disease is producible, but 

 with cultures the experiment has not yielded positive 

 results ; it ought to be noted, however, that the 

 cultures used in Experiment 6 were of considerable 

 age, and it must be added that the second feeding 



