126 THE GROUSE DISEASE chap. 



forms a continuity with, the inflamed central marrow, 

 this showing its blood-vessels much distended by 

 blood and its tissue a continuous mass of round-cells. 

 From the places of greatest inflammation, i.e. at the 

 ends of the shaft, the engorgement of the vessels and 

 the infiltration with leucocytes of the bone and the 

 central marrow extend a little way beyond. This 

 condition of periostitis and osteomyelitis is present in 

 all the cases which I examined, and the rarefaction 

 and absorption of the bone at the end of the shaft and 

 the subsequent dissociation is hereby easily explained. 

 In cover-glass specimens made of the inflamed 

 tissues, and in sections through the inflamed parts, a 

 few minute oval or cylindrical bacilli are constantly 

 met with (they require for demonstration prolonged 

 staining of the sections — a couple of hours and more 

 in methyl blue aniline water ; or staining for half an 

 hour to an hour in gentian violet aniline water shows 

 the bacilli well). Of course it is necessary after the 

 staining to well decolorise the section in methylated 

 spirit in order to remove as much as possible the dye 

 from the tissue cells, the bacilli retaining the dye more 

 persistently. From several cases cultivations were 

 made ; with sterile scissors the bone is laid bare, with 

 another pair of sterile scissors an incision is made into 

 the inflamed part, then with a sterilised platinum loop 

 a small particle of the marrow is removed and trans- 



