128 THE GROUSE DISEASE chap. 



crenate. In stab cultures the line of inoculation 

 becomes marked as a grey line made up of separate 

 droplets. The gelatine is never liquefied by the 

 growth. 



In fresh specimens made of the colonies, i.e. ex- 

 amining a particle of the growth in sterilised salt 

 solution or in broth, the bacilli are without motility ; 

 in such specimens, as also in cover-glass specimens 

 dried and stained, the microbes are short bacilli 

 rounded at their ends, many are short ovals, some 

 cylindrical ; there are always some cylindrical and 

 rod-like bacilli present which are thicker in the middle 

 portion than at the ends, they look, therefore, spindle- 

 shaped ; they occur singly or often as dumb-bells. In 

 specimens made of gelatine cultures the bacilli differ 

 somewhat from those grown on Agar, inasmuch as in 

 the former most of the microbes are short ovals, and 

 in stained specimens show at each end in the trans- 

 parent sheath a stained granule. These look very much 

 like the typical fowl cholera bacilli ; but also in speci- 

 mens of gelatine culture there are some which are 

 rod-shaped or even distinctly cylindrical ; but not so 

 numerous by any means as in Agar cultures. In 

 these latter the majority are rod-shaped or cylindrical, 

 and amongst them occur such as are characteristically 

 spindle-shaped ; but also in Agar cultures there occur 

 a good many that are short ovals. Figs. 52 and 53 



