XIV ''CRAMPS" IN YOUNG PHEASANTS 129 



show well the differences between the bacilli in gela- 

 tine and Agar cultures respectively. 



Measurements made in dried and stained cover- 

 glass specimens give the following numbers : thick- 

 ness about 0.3 micro-millimetres ; there is no differ- 

 ence in this respect between the bacilli grown on 

 gelatine and those on Agar ; the cylindrical spindle- 

 shaped bacilli are thickest and measure as much as 

 0.4 to 0.5 /A in the thickest portion ; the length of the 

 majority of the bacilli from gelatine cultures is 0.4 /i to 

 0.6, a few 0.8 /i, very few more than this — 1.6 ix. In 

 Agar cultures the majority are 0.6 to 0.8 to i.o /x long, 

 some up to 1.6 /i. The cultures and sub-cultures which 

 I prepared from the young pheasants in June and 

 July 1887, and which I described in the Field, July 

 30, 1887, I put away^ during the following vacation, 

 August and September, in order to resume the inquiry 

 on my return towards the latter half of September, 

 but unfortunately, to my great discomfiture, they were 

 all found dead ; every tube put aside, though it re- 

 mained free of all accidental growth and looked in 

 all respects preserved, was tested by sub-cultures, but 

 with no result ; none could be started from them, and 

 therefore all further experimentation with them came 

 to an end. I have not been able hitherto to procure 



1 A few experiments on guinea-pigs and mice by subcutaneous 

 inoculation of culture did not yield any definite result. 



K 



