(Triacid Stain.} 



i, 2, 3, 4. Small Lymphocytes. 



Contrast the faintly colored protoplasm of these cells in the triple stained specimen 

 with their intensely basic protoplasm in the film stained with eosin and methylene- 

 blue, 17 and 18. The cell body of i is invisible. Note the kidney-shaped nucleus 

 in 4. 



5, 6. Large Lymphocytes. 



With this stain the nucleus reacts more strongly than the protoplasm; with eosin 

 and methylene-blue (19, 20), on the contrary, the protoplasm is so deeply stained 

 that the nucleus appears pale by contrast. This peculiarity is also observed in 

 the smaller forms of lymphocytes. 



7, 8. Transitional Forms. 



Note the moderately basic and indented nucleus, and the almost hyaline non- 

 granular protoplasm. Compare 8 with the myelocyte, 7, Plate I, these cells 

 differing chiefly in that the myelocyte contains neutrophile granules. 



9, 10, ii. Polynuclear Neutrophiles. 



These cells are characterized by a polymorphous or polynuclear nucleus, sur- 

 rounded by a cell body filled with fine neutrophile granules. In 11 the nuclear 

 structure is obviously separated into four parts; in 9 it is moderately, and in 10 

 markedly, polymorphous. 



12, 13. Eosinophiles. 



The nuclei are not unlike those of the polynuclear neutrophile, except that they 

 are somewhat less convoluted, and poorer in chromatin, staining less intensely. 

 The protoplasm is filled with coarse eosinophile granules, the characteristics of 

 which are clearly illustrated by 13, a "fractured" eosinophile. 



14. Eosinophilic Myelocyte. 

 Compare with 15. 



15, 1 6. Myelocytes. (Neutrophilic.) 



These cells are morphologically similar to 14, except that they contain neutrophile 

 instead of eosinophile granules. Note that the granules of the myelocyte are 

 identical with those of the polynuclear neutrophile. A dwarf form of myelocyte 

 is represented by 16. 



(Eosin and Methylene-blue.) 



17, 18. Small Lymphocytes. 



Note the narrow rim of pseudo-granular basic protoplasm surrounding the nucleus, 

 and the pale appearance of the latter. 



19, 20. Large Lymphocytes. 



Budding of the basic zone of protoplasm is represented by 20. Both of these 

 cells belong to the same type as 5 and 6. 



21, 22. Large Mononuclear Leukocytes. 



Compared with 19 arid 20, these cells have a decidedly less basic protoplasm, but 

 a somewhat more basic nucleus. In the triple stained film these differences can- 

 not be detected, so that they must be classed as large lymphocytes. 



23. Transitional Form. 



The distinction between this cell and 24 is not marked; the nucleus of the latter 

 simply being somewhat more basic and convoluted. 



24, 25, 26, 27. Polynuclear Neutrophiles. 



With this stain these cells show a feebly acid protoplasm, and lack granules. 

 Note that the more twisted the nucleus the deeper it is stained. Compare with 

 9, 10, and n. 

 28, 29. Eosinophiles. 



Compare with 12 and 13. 



30. Eosinophilic Myelocyte. 

 Compare with 14. 



31. Basophile. (Finely granular.) 



This cell is characterized by the presence of exceedingly fine ^-granules, staining 

 the pure color of the basic dye. The nucleus is markedly convoluted and deficient 

 m chromatin. The cell here shown was found in normal blood 



32. 33. 34, 35, 36- Mast Cells. 



The granules take a modified basic color, as shown by their royal-purple tint in 

 this illustration. Note their unusually large size and ovoid shape ^35 Se5 

 peculiar distribution in 35 and 36, and their irregularity in size in 32 and 36. 

 With the tnacid mixture these granules, as well as those of the finely granular 

 basophile 31, remain unstained, showing as dull-white stippled areas in the cell 

 ody The nuclear chromatin of the mast cell is so delicate and so feebly stained 



