396 



BLOOD AND LYMPH. 



of oxyhemoglobin (less than 0.01 per cent.) the /5-band is lost and 

 the a-band is very faint in layers one centimeter thick. With 

 stronger solutions the bands become darker and wider and finally 

 fuse, while some of the extreme red end and a great deal of the violet 

 end of the spectrum is also absorbed. The variations in the absorp- 

 tion spectrum, with differences in concentration, are clearly shown 

 in the accompanying illustration from Rollett * (Fig. 171) ; the thick- 



66 



....L.1.A..J J .I.J I Lul 



Fig. 170. Table of absorption spectra (Ziemke and Mutter) : 1, Absorption spectrum 

 of oxyhemoglobin, dilute solution ; 2, absorption spectrum of reduced hemoglobin ; 3, ab- 

 sorption spectrum of methemoglobin, neutral solution ; 4, absorption spectrum of met- 

 hemoglobin, alkaline solution ; 5, absorption spectrum of hematin, acid solution ; 6, ab- 

 sorption spectrum of hematin, alkaline solution. 



ness of the layer of liquid is supposed to be one centimeter. The 

 numbers on the right indicate the percentage strength of the oxyhem- 

 oglobin solutions. It- will be noticed that the absorption which 

 takes place as the concentration of the solution increases affects 

 the red-orange end of the spectrum last of all. 



Solutions of reduced hemoglobin examined with the spectroscope 

 * Hermann's "Handbuch der Physiologic," vol. iv, 1880. 



