26 PLANT ENZYMES [ch. hi 



(B) Action of the glucoside-splitting enzyme. (Caldwell and Courtauld, 9 ; Henry 

 and Auld, 13.) This enzyme will act upon the glucoside, amygdalin, which is present 

 in bitter almonds, with the production of glucose, benzaldehyde and prussic acid 

 (see p. 160). Into two small flasks (a) and (6) put the following: 



(a) 20 c.c. of a 2 7o solution of amygdalin + 20 c.c. of the filtrate from zymin. 



{h) 20 c.c. of the same solution of amygdalin + 20 c.c. of the boiled filtrate from 

 zymin. 



Add a few drops of toluol to both flasks and then cork, inserting, with the cork, 

 a strip of paper which has been dipped in solutions of picric acid and sodium carbonate 

 (see p. 161). Put both flasks in an incubator at 38° C. for 12-24 hours. The picrate 

 paper in flask {a) will have reddened. Add a little Fehling's solution to the liquid in 

 the same flask and boil. The Fehling will be reduced. The liquid in flask (6) will 

 only reduce Fehling slightly [see Expt. A (6)] and the picrate paper will not be 

 reddened. 



REFERENCES 



Books 



1. Abderhalden, B. Biochemisches Handlexikon, v. Berlin, 1911. 



2. Bayliss, W. M. The Nature of Enzyme Action. London, 1919. 4th ed. 



3. Euler, H. General Chemistry of the Enzymes. Translated by T. H. Pope. 

 New York and London, 1912. 



4. Harden, A. Alcoholic Fermentation. London, 1914. 2nd ed. 



5. Vernon, H. M. Intracellular Enzymes. London, 1908. 



6. Wohlgemuth, J, Grundriss der Fermentmethoden. Berlin, 1913. 



Papers 



7. Armstrong, H. B., and Armstrong, B. P. The Origin of Osmotic Effiects. 

 III. The Function of Hormones in Stimulating Enzymic Change in Relation to 

 Narcosis and the Phenomena of Degenerative and Regenerative Change in Living 

 Structures. Proc. R. Sac, 1910, B Vol. 82, pp. 588-602. Ibid. IV. Note on the 

 Differential Septa in Plants with reference to the Translocation of Nutritive Materials. 

 Proc. R. Soc, 1912, B Vol. 84, pp. 226-229. 



8. Armstrong, H. B., and Armstrong, B. P. The Function of Hormones 

 in regulating Metabolism. Ann. Bat., 1911, Vol. 25, pp. 507-519. 



9. CaldTvell, R. J., and Courtauld, S. L. Studies on Enzyme Action. 

 IX. The Enzymes of Yeast: Amygdalase. Proc. R. Soc, 1907, B Vol. 79 

 pp. 350-359. 



10. Harden, A. The Enzymes of Washed Zymin and Dried Yeast. I. Car- 

 boxylase. Biochem. J., 1913, Vol. 7, pp. 214-217. • 



11. Harden, A., and Norris, R. V. The Enzymes of Washed Zymin and 

 Dried Yeast. II. Reductase. Biochem. J., 1914, Vol. 8, pp. 100-106. 



12. Harden, A., and Zilva, S. S. The Enzymes of Washed Zymin and Dried 

 Yeast. III. Peroxydase, Catalase, Invertase and Maltase. Biochem. J., 1914, Vol. 8, 

 pp. 217-226. 



13. Henry, T. A., and Auld, S. J. M. On the Probable Existence of Emulsin 

 in Yeast. Proc. R. Sac, 1905, B Vol. 76, pp. 568-580. 



