140 PROTEINS AND AMINO- ACIDS [ch. 



the free state; combined with a small amount of acid as a salt, it is 

 insoluble in water but soluble in neutral salt solution, that is, it has the 

 solubilities of a globulin (see p. 147). 



Expt. 130. The formation of salts hy edestin. Grind up 5 gms. of seeds of the 

 Hemp {Cannabis saiiva) in a coffee-mill. Extract with 50 c.c. of warm (not above 

 60° C.) 10% sodium chloride solution and filter. Add a drop of strong hydrochloric 

 acid to the filtrate. Edestin chloride, which is insoluble in salt solutions, is precipi- 

 tated. Filter and drain off all the liquid, wash once and then suspend the precipitate 

 in distilled water. Add 1 or 2 drops of hydrochloric acid carefully and stir till most 

 or all of the precipitate goes into solution. Filter, and to the filtrate add a few drops 

 of saturated sodium chloride solution. The edestin acid salt is again precipitated. 



The following is a list of the principal known globulins (Osborne, 2): 



Pea (Pisum sativum). 



Legumin, in seeds of 



Broad Bean {Vicia Faha). 

 Vetch ( Vicia sativa). 



I Lentil (Ervum Lens), 



Vignin, in seeds of Cow Pea ( Vigna sinensis). 



Glycinin, in seeds of Soy Bean {Glycine hispida). 



r Kidney Bean (Phaseolus vulgaris). 

 Phaseolin (crystalline), in seeds of -| Adzuki Bean (P. radiatus). 



'^Lima Bean (P. lunatus). 

 Conglutin, in seeds of Lupin (Lupinus). 



rPea (Pisum sativum), 

 Vicilin, in seeds of ^ Broad Bean ( Vicia Faha). 



ILentil (Ervum Lens). 

 Corylin, in seeds of Hazel Nut (Corylus Avellana). 



'Almond (Pruniis Amygdalus). 



Amandin, in seeds of 



Peach (P. Persica). 

 Plum (P. domestica). 



, Apricot (P. Armeniaca). 



^European Walnut (Juglans regia). 

 Juglansin, in seeds of - American Black Walnut (/. nigra). 



lAmerican Butter-nut (J. cinerea). 

 Excelsin (crystalline), in seeds of Brazil Nut (Bertholletia excelsa). 



Edestin in seeds of. Hemp (Cannabis sativa). 



Avenalin, in seeds of Oat (Avena sativa). 



Castanin, in seeds of Sweet Q\iQ^tnVit(Gastaneavulgaris). 



Maysin, in seeds of Maize (Zea Mays). 



Tuberin, in tubers of Potato (Solanum tuberosum). 



