44 Gytolysin and Agglutinin 



took place between venom and ricin. The haemolysin in venom resisted 

 an exposure of 30 minutes at 75 80 C., being slightly reduced in 

 power after 15 minutes at 100 C. Venom agglutinins exposed for 

 30 minutes at 7580 C. were destroyed. 



Dubois (25, ix. 1902) considers the agglutinins and immune-bodies 

 of artificially haemolytic sera to be distinct, although they behave 

 similarly in their resistance to heat, putrefaction, light, etc. He states 

 that he was able to separate them by repeatedly injecting rabbits, 

 (a) with intact washed fowl's corpuscles, and (b) with the same corpuscles 

 previously heated to 115 C. for 15 minutes. The fresh serum of the 

 rabbit receiving treatment (a), agglutinated and haemolysed fowl's 

 corpuscles, whereas the second (6) did not haemolyse, although it 

 contained some agglutinin. Dubois cites Defalle as having found that 

 certain germs (B. typhosus, B. mycoides, B. mesentericus, etc.) when heated 

 to 115C., and injected into animals, produced an agglutinative serum 

 not containing immune-body, whereas the latter is readily obtained by 

 treating animals with unheated germs. 



