Production of Antisera 



53 



Uhlenhuth (15, XI. 1900) was able to obtain antiscnun in a rabbit 

 fed on egg-white, the solution being intn>duml daily by means of a 

 sound, and the precipitin appearing after the feeding had been con- 

 tinued 24 days. Similarly Michaelis and Oppcnheimer (1902, p. 355) 

 fed rabbits with ox scrum in large quantities, and obtained antiserum 

 for ox blood. They attribute the formation of antiserum, through 

 excessive feeding with hcterologous albumen, to the escape of some of it 

 from the action of the peptic digestion, for precipitins for milk, etc. are 

 not found in the serum of normally fed man. 



In the following table I have summarized the methods of treatment 

 of rabbits adopted by different workers, the data in some cases being 

 rather incomplete. 



* Gave reaction with 1 : 1000 blood dilutions. 



t Milk sterilized 90 minutes at 65 C., injected every 4 days. 



J Contained 1 2 g. of albumen per litre. 



Uhlenhuth made injections every 5 6 days, Stern every two days, 

 Wassermann and Schiitze every 3 4 days, others at intervals which 

 are generally not stated. In Bordet's experiments (in. 1899, p. 240, 

 not cited in the table), the milk was partially sterilised at 65 C. prior 

 to injection intraperitoncally. Wassermann and Schiitze sterilized it 

 by means of chloroform. 



Without making an attempt at completeness, the above table very 

 clearly shows a great divergence in the mode of treatment, more especially 

 with regard to the duration of treatment and the amounts of substance 



