Immune Bodies in Antisera 



95 



from guinea-pigs treated with heated rabbit and dog serum. He found 

 that all these antisera contained immune body, which for instance 

 in lactoserum attached itself to milk, and thus allowed the precipitin to 

 act, just as is the case with the anti-microbic and other sera above 

 referred to. He tested this as follows: 

 He prepared 



LS. Fresh lactoserum heated to 56 C. for 30 minutes. 

 NS. Normal rabbit serum heated to 56 C. for 30 minutes. 

 A. Normal rabbit serum 24 hours old, containing alexin. 

 C. Washed fowl's corpuscles suspended in saline after having been 

 subjected to action of heated immune-serum of a rabbit which had 

 received injections of fowl's corpuscles. 

 L. Cows' milk. 

 NaCl. Normal salt solution. 



He mixed these in different ways as follows (I have omitted the 

 quantities intentionally) : 



Test I. mixed L 

 LS 



A j 



II. mixed L 



NS 

 A 



III. mixed NaCl 



LS 

 A 



IV. mixed NaCl 



NS 

 A 



V. mixed L 

 LS 

 NaCl 



VI. mixed L 



NS 

 NaCl J 



result : precipitation ; fluid added to C don't haemolyse, acts 

 as if no alexin were present ; alexin must be attached to 

 milk as it was not anchored to LS in Test III., nor by 

 milk alone in Test II. 



nQ preci p itat j oni Q haemolised as in Tests III. and 

 IV., alexin being free. 



result : haemolised C (added 24 hrs. later) in 30 minutes, 

 completely in \\ hrs. In this and Test IV. the 

 milk being absent, the alexin remained free to act 

 on the sensitized corpuscles. 



result: no haemolysis of C, there being no alexin 

 present. 



Gengou concludes from the above ingenious experiment that lacto- 

 serum contains immune body (" sensibilisatrice ") besides the precipitin. 



