Effects on different Albumins 109 



Michaelis (9, x. 1902, p. 735) injected horse and ox serum-albumin 

 into rabbits, and found the antisera to precipitate both serum-albumin 

 and globulin. Contrary to Nolf he found, possibly owing to his having 

 used different methods, that serum-albumin injections lead to the 

 formation of precipitins which acted on serum-albumin. Gengou 

 (25, X. 1902, p. 746), experimenting in another manner (see Immune 

 Bodies in precipitating Antisera, p. 94) and using antisera obtained 

 from rabbits treated with dog serum, found that anti-dog serum acting 

 on dog euglobulin and serum-albumin led to precipitation and fixation 

 of complement. Lactoserum, acting on cow casein and lactoglobulin 

 did likewise, whereas lactalbumin was not precipitated and complement 

 remained free. This experiment, often repeated, he considers contradicts 

 the statement of Hamburger (see p. 107). Schiitze (6, xi. 1902, p. 805) 

 found haematosera to also precipitate dilutions of the homologous sper- 

 matozoa of different animals, the spermatozoa tested being those of 

 the horse, ox, sheep, pig, and man. Uhlenhuth (1901, p. 501) had 

 previously noted that haematosera also clouded spermatozoa solutions 

 of the same animal. Ziemke (15, ix. 1902) appears to have obtained 

 results which do not point to the specificity of the reaction. 



Falloise (25, xi. 1902, p. 836) refers to the discrepancies in the 

 results of other workers as being possibly due to their different method 

 of treating their animals. Working with the sera of the ox and horse, 

 Falloise separated the globulin and albumin after the methods of 

 Leblanc and Nolf, whose results, as I have noted above, are not in 

 accord. The results obtained with anti-globulin and anti-albumin for 

 ox and horse confirmed each other, for Falloise found that, A, anti-horse 

 globulin precipitated normal horse serum and globulin solution, albumin 

 solutions being very slightly affected. It exerted a very slight action 

 on ox serum, and globulin, none on ox albumin. (Anti-ox serum 

 behaved in a corresponding manner upon ox and horse serum etc.) 

 B, Anti-horse serum-albumin produced slight reactions and deposit 

 in horse serum, in albumin and globulin solutions. With ox serum 

 and globulin solutions there was only a very slight trace of deposit 

 in 24 hrs., no effect on ox serum albumin solutions. (Anti-ox serum- 

 albumin behaved in a corresponding manner towards its homologous 

 serum, etc.) 



Falloise would explain the failure of albumin to produce precipitin 

 in larger amount to the presence of but little globulin therein, attributing 

 the precipitin formation entirely to the action of globulin which it 

 contains in consequence of our imperfect method of purification. He 



