204 Tests with Precipitins 



Carinatae. Here we also observe faint cloudings occurring in dilutions 

 of egg-white both of Ratitae (Emu) and Carinatae. A slight action 

 would seem to be exerted upon reptilian bloods, all other non-avian 

 bloods giving practically negative results. 



B. Tests with Antisera for Avian Egg-White. 



(1) Anti-fowl's Egg. 



Myers (14, vn. 1900) treated rabbits with crystallized egg-albumin 

 of the fowl and obtained an antiserum which precipitated the egg- 

 albumin of the fowl, and to a slight extent that of the duck. No 

 reaction was obtained when the antiserum was added to normal rabbit, 

 bullock, or sheep blood dilutions. Subsequently Uhlenhuth (15, XI. 

 and 1, xil. 1900) injected fowl egg-white in salt solution intraperitoneally 

 into rabbits, and obtained a powerful antiserum, which produced re- 

 actions with egg-white diluted to 1 : 100,000, and weaker reactions 

 with pigeon egg than with that of the fowl. He concluded from 

 this that the albuminous constituents of both species of eggs are 

 closely allied. Mertens (14, HI. 1901) confirmed the foregoing results. 

 Uhlenhuth (25, IV. 1901) reported later that he had obtained a powerful 

 anti-egg serum which acted on fowl blood dilutions, and produced 

 a slight cloud in goose blood. He obtained an immediate precipitation 

 upon adding the antiserum to 2'5/ fowl blood dilution. It produced 

 almost as powerful reactions with egg-white dilutions of goose, duck, 

 guinea-fowl, though less with the pigeon's than with its homologous (fowl) 

 egg-white. A rabbit treated with goose egg yielded a serum which 

 produced a precipitum in goose blood, less clouding in fowl blood, 

 dilutions. It gave a great and immediate precipitation with goose and 

 duck, a considerable clouding with fowl, guinea-fowl, and pigeon eggs. 

 He concludes it will not be possible to differentiate eggs as has been 

 possible for bloods. 



Nuttall (16, xn. 1901, p. 408) found anti-fowl egg serum, when 

 powerful, to produce a reaction with fowl blood, but never so intense an 

 action as upon its homologous substance. Levene (21, xn. 1901) treated 

 animals for two months with egg-white and found their serum to 

 precipitate egg-albumin, egg-globulin, yolk of egg and fowl and turkey 



