Anti-FowTs Egg-White 205 



serum. Nearly all the preceding observers state that normal rabbit or 

 other sera produce no such effect on the dilutions, these being used for 

 control purposes. 



Nuttall (20, i. 1902) reported that this antiserum produced clouds 

 with a variety of avian bloods (parrot, swan, heron, stork, conure, crow, 

 emu) also with egg-white of emu, and in addition with blood dilutions 

 of Alligator sinensis, A. mississippiensis, Chelone midas, and Testudo 

 ibera, and it might therefrom appear " that the egg possesses a vestige 

 of reptilian character." In view of the continued negative results 

 with other bloods I referred to this observation (5, iv. 1902) as "very 

 suggestive in view of the reptilian origin of birds," and the reaction as 

 possessing possibly a " reptilian-avian " character. Dr Graham-Smith 

 has since been pursuing investigations in this direction, and reports 

 upon them in Section VIII. 



Pursuing a different line of research (see p. 94) Gengou (25, x. 1 902, 

 p. 750) found he was unable, according to his method, to find any 

 difference in the action of anti-fowl egg serum as tested upon eggs of 

 the fowl, turkey, pigeon, and duck. He was unable (p. 753) to deter- 

 mine that anti-egg serum possessed an action on fowl blood. As we 

 have seen, anti-egg only acts upon the corresponding blood when 

 powerful. Finally Oberrnayer and Pick (1902) remark that fowl serum 

 pseudoglobulin contains a body which reacts to anti-egg (from rabbit) 

 in a similar manner to egg-white. 



789 Tests with Anti- Fowl's Egg. 



The following tests were conducted with two antisera obtained from 

 two rabbits treated with the white of egg of the domestic fowl. The 

 one antiserum was moderately powerful, the other exceedingly powerful. 

 The antisera were standardized, but I should estimate the precipitum 

 of the stronger antiserum at '06 c.c. 



In this table it will be seen that the largest reaction occurs with the 

 egg-white of the fowl, several eggs having been tested with the same 

 result. Second-class reactions were obtained with another carinate egg, 

 with the egg of the emu, with the blood of a heron, and the blood of 

 two Reptilia (Chelonia and Crocodilia). Otherwise all the medium 

 and slight cloudings occurred amongst the bloods of Aves and Reptilia, 

 if we except a single slight clouding in one out of 388 mammalian 

 bloods tested. 



