Anti-Frog, Fish and Lobster Sera 211 



The foregoing table shows that the antiserum for Rana temporaria 

 only produced a large reaction in the blood dilutions of this species, and 

 that it also produced a reaction with the blood of Rana tigrina, the 

 results with all other bloods being absolutely negative. 



In my paper of 5, iv. 1902 I reported upon tests made on 508 



bloods with this antiserum, these bloods being now included in the 



above table. The only investigator who has worked with anti-amphibian 



sera is Philippson (about Sept. 1902), who confined himself to a few 



tests. He cites my paper, and states that he has been able to 



confirm my results. He injected 1 c.c. of defibrinated frog's blood (Rana 



viridis var. esculenta) into a rabbit, making two such injections with 



a week's interval. He found his antiserum to precipitate the serum of 



Rana viridis var. typica, and that of R. fusca, but it had no effect on 



that of Hyla arborea, Bufo mdgaris and Salamandra maculosa. The 



antiserum had also no effect upon the serum of the pig or calf. I would 



remark that his antiserum must doubtless have been weak after only 



making two injections and, what is more, introducing such small amounts 



of frog blood. In my experience, I have not noted the presence of pre- 



cipitins in the serum of animals prior to the third injection, and then 



only in small amount. I did not however make a note as to the earliest 



period at which my rabbit showed precipitin when treated with frog's 



blood. 



XI. Antisera for bloods of Pisces. 



Tchistovitch (v. 1899) made his fundamental discovery upon the 

 blood precipitins by treating the rabbit, guinea-pig, goat, or dog with 

 eel serum. Tests upon different fish bloods are being made the subject 

 of study by Dr Graham-Smith at my suggestion. The results should 

 be of considerable interest. With the exception of de Lisle (xi. 1902, 

 p. 399) who, following the method of Tchistovitch, also obtained anti-eel 

 serum from rabbits, no further work has as yet been done. De Lisle 

 (p. 403) obtained precipitins by injecting either eel serum or eel 

 corpuscles. 



XII. Antiserum for bloods of Crustacea. 



450 Tests with Anti-Lobster Serum. 



These tests were made with one antiserum derived from a rabbit 

 treated with the blood of the lobster (Homarus vulgaris). The anti- 

 serum was fairly powerful. 



14 2 



