322 Quantitative Precipitin Tests 



No trace of reaction was obtained with corresponding dilutions of 

 the bloods of Man, Cat, Hyaena, Dog, Seal, and Wallaby. Further 

 investigations will be required with antisera for Insectivora. 



3. Quantitative Tests with Anti-Carnivore Sera. 



Tests with Anti-Gat Serum (1. vi. '02). 

 Antiserum from rabbit treated with the serum of Felis domesticus. 



Precipituin 

 Blood of Date amount Percentage 



739 Felis domesticus 8. v. 02 "005 100 another cat's serum gave an 



identical result 



579 Lutra vulgaris 4. iij. 02 -002 40*f gee tex( . 



367 Ursus tibetanus 27. xii. 01 -001 20") 



738 Felis tigris 21. v. 02 -001 20 another tiger (103) gave !<;"/ 



reaction 

 Canis familiaris -0008 10 



The antiserum used in this case was weak. The only bloods which 

 gave measurable quantities of precipitum were those from Carnivora. 

 The blood of Felis caracal (300) gave no precipitum, this being probably 

 ' due to an excess of chloroform having been added to it, causing some 

 precipitation ; it however reacted with a distinct cloudiness upon the 

 addition of antiserum. The bloods of the Jackal, Raccoon and Genetta 

 gave no reaction. The high figures given by the bloods of Lutra* 

 and Ursus* are attributable to these bloods being somewhat concen- 

 trated through keeping, as noted elsewhere. Owing to the very 

 small amount of precipitum with the bloods of Ursus, Canis, and 

 Felis tigris, these measurements cannot be considered accurate. Of 

 11 bloods of Carnivora examined with this weak antiserum, seven 

 gave a precipitum, one a clouding only, and three a negative result. 

 Twenty-eight other mammalian bloods were tested at the same time 

 with uniformly negative results, the bloods examined being from 

 Primates (7), Ungulates (12), Cetacea (1), Marsupialia (8). 



