328 Quantitative Precipitin Tests 



Tests with Anti-Pig Semm (18. II. '02). Series I. 



Antiserum from rabbit treated with the serum of Sus scrofa 

 (domesticated Pig). 



The figures given here were published in part by Nuttall (5, iv. 1902) 

 in the British Medical Journal. Of five normal sheep sera four gave 

 a precipitum of '006, one of '0055 with this antiserum, the sheep having 

 been slaughtered 20 hours previously. The reactions obtained were as 

 follows : 



I'recipituin 

 amount Percentage 



045 100 



0075 16 



0065 14 



006 13 



006.5 14 



006 13 



0025 5 ' 



1 It may be noted that such a reaction was only obtained with a marsupial blood with 

 two very powerful anti-pig sera (Series I and III) whereas in Series II less precipitum was 

 obtained although an apparently even more powerful antiserum was used. Series II was 

 tested 4 months later than Series I. The most powerful anti-sheep serum (Series I) also 

 gave a 5 % reaction with a marsupial blood. 



It will be noted that the antiserum in this case was very powerful 

 (precipitum of '045). It produced an immediate reaction with pig 

 serum dilution, a deposit being formed, and the supernatant fluid clear 

 within 80 minutes. In the other bloods the clouding took place more 

 slowly as also did deposition. The Wallaby blood dilution only began 

 to show faint clouding after 30 minutes. This antiserum demonstrates 

 the " mammalian reaction," and does not show any distinct difference 

 between the behaviour of the other non-homologous bloods if we except 

 that of the Wallaby. 



Tests with Anti-Pig Serum (11. vi. '02). .Series //. 



In this series another antiserum to the preceding was tried, being 

 obtained in the same way from a rabbit. In this case a larger series 

 of bloods was tested, with the following results: 



