(5) Tests with Anti-Crane' 's-Egg Serum. 



This antiserum was prepared by intraperitoneal injections of the 

 egg-albumin of the Cape Crowned Crane (Balearica regulormri) into 

 a Rabbit. 



Percentage 



Material tested .? X + + "reasons" 

 27 Kijg- Albumins 



(o) 15 Birds' egg-albumiDs 4 . 2(13"/,,) 5 (33"/ u ) 4(2"/) TA 



(b) 2 Reptile 2 -0 



(c) 3 Amphibian ,, 3 . . . Q 



(d) 7 Fish 7 . . . o 

 129 fi/oorf Sera 



as Chelonia 13 2 (13/,,) . . . 13 



I 4 Crocodilia 4 . . Q 



60 Beptiliaj 13 Lacertilia 18 . . . . . Q 



^28 Ophidia 28 . . . o 



18 Amphibia 18 . . . Q 



39 Pisces 39 . . . o 



12 Arthropoda 12 . . o 



This was a rather weak antiserum and failed entirely to react with 

 four birds' egg-albumins, and gave only two very feeble reactions with 

 two tortoise bloods. 



B. Qualitative tests with Antiserum for Fowl's Blood. 



This antiserum was prepared by Dr Nuttall, and with it only the 

 following tests on birds' egg-albumins were made to render the experi- 

 ments already carried out more complete. 



1'ercentAgi; 



of jwsitive 



Material tested * ' * + reactions 



13 Birds' egg-albumins 3 (23/,,) 1 (8 11 /,,) 5 (38%) 2(14%) 2 (14''/ ) . 77 



Summary of results of Qualitative Tests with Antisera to Avian 



Egr/-Albiiinii>t>. 



With the five antisera for Avian egg-albumins u'ti tests were made 

 with dilutions of birds' egg-albumins and 85% of distinct, positive 

 reactions were obtained, of which 62% were very well marked. < >nr 

 egg-albumin dilution, namely that of the chaffinch, failed (<> react, with 

 any of these antisera, but reacted with antiserum to fowl's blood. 



